To examine if different sources of tissue culture plastics has any effects on the growth and proliferation of Human fibrosarcoma HT 1080 cell line.

Abstract

The design of this experiment was conducted in order to analyse the effect of tissue culture plastic surface on Human fibrosarcoma HT 1080 cell line and to establish the optimal plastic wares for this cell line growth and proliferation, which is still lacking even it is commonly used in vitro studies. HT 1080 cell line is strongly recommended as a gold standard for the Lentivirus titration. In this context, it appears especially interesting to which extent the HT 1080 cell line proliferation depends on which type of tissue culture plastic plates were used in any experiment. In this study we optimized the growth and the proliferation of the HT1080 cell line by growing them in three different 96 wells tissue culture plates including Falcon, Coming and Greiner, and study the cells proliferation using XTT assay (Roche based). Thus we considered the HT 1080 cell line proliferation curve obtained on 2 weeks time and we investigated the proliferation influence of this cell line seeded in 3 different plastic plates. We found that falcon tissue culture plastic were could be more widely considered as a potential plastic ware tool for growing HT 1080 cell line from proliferation curves obtained under 3 different experimental plastic plates. Falcon tissue culture plate was more likely suitable plastic plate to be used in seeding the HT 1080 cell line.