Comparative identification of Burkholderia species and Pseudomonas species
Cardiff Metropolitan University
MetadataShow full item record
Cystic Fibrosis (CF) is a common recessive genetic disease that can affect the entire body, but especially the lungs. It causes progressive disability and often early death. The defect in the gene disturbs the sodium and chloride transport within epithelial cells leading to the accumulation of abnormally thick mucus. This mucus forms the ideal breeding ground for many bacteria, which leads to many complications for CF patients. The most common bacteria found in the lungs of CF patients are Burkholderia cepacia complex, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. Accurate identification of these pathogens is vital in patients with CF as a positive result has serious social, clinical and organisational consequences. A number of methods can be used to identify these organisms including, selective agars, automated systems, such as Phoenix, and molecular PCR. This study compares the ability of the multiplex PCR and the automated Phoenix system to identify to species level 40 characterised isolates including B. cepacia complex, P. aeruginosa, and S. maltophilia. An automated system wrongly identified 7/30 B. cepacia complex and P. aeruginosa isolates. Multiplex PCR correctly identified 19/20 and 9/9 for these organisms respectively. However, identification of .S. maltophilia was better on the Phoenix as problems were observed for this organism when performing PCR.
BSc (Hons) Biomedical Science