The role of conjugated linoleic acid in regulating CD206 expression in monocytic cells.
Cardiff Metropolitan University
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The role of dietary factors in the prevention of atherosclerosis has been the focus of important attention. The classic example is conjugated linoleic acids CLA are a mixture of isomers of linoleic acid found mainly in the meat of ruminant animals and dairy products. The exact mechanism in which CLA effects in human is not yet clear this is because most of the experiments based on animal models. Activation of nuclear receptor PPARy is linked to the beneficial effects of inflammation, atherosclerosis, Type 2 Diabetes T2D and lipid homeostasis. These beneficial effects include differentiating circulating monocytes toward M2 macrophages (anti-inflammatory phenotype). Previous researches have shown that the dietary lipid CLA present in dairy products can trigger the nuclear transcription factor PPARy which has been shown to regulate monocyte polarisation towards the beneficial M2 form. In-vitro differentiation of monocytes into the M2 phenotype by CD206 expression was achieved by treated THP-1 monocytic cells with PMA and incubated up to 72 hours with the Th2 cytokine IL-13 (15ng/ml), the dietary lipid CLA (20 and 100µM) and a specific PPARy ligand Rosiglitazone (1µM). CLA significant increased the expression of CD206 in M2 polarized macrophages with presence of IL-13. Tumour necrosis factor alpha TNFα was inhibited by different concentration of CLA and probably down regulated by IL-13. The level of IL-8 cytokine was increased after treating THP-1 cells with CLA and Rosiglitazone. In conclusion, this study presents evidence that the dietary lipid CLA can induce monocyte polarisation towards a beneficial M2 phenotype partly during their ability to activate PPARy, thus may suggest constitute a benefits as it has anti-inflammatory properties.
BSc (Hons) Biomedical Science
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