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dc.contributor.authorYakeu, G.
dc.contributor.authorButcher, Lee
dc.contributor.authorIsa, S.A.
dc.contributor.authorWebb, Richard
dc.contributor.authorRoberts, Aled W.
dc.contributor.authorThomas, Andrew
dc.contributor.authorBackx, Karianne
dc.contributor.authorJames, Philip
dc.contributor.authorMorris, Keith
dc.date.accessioned2014-04-30T08:19:04Z
dc.date.available2014-04-30T08:19:04Z
dc.date.issued2010
dc.identifier.citationYakeu, G., Butcher, L., Isa, S., Webb, R., Roberts, A.W., Thomas, A.W., Backx, K., James, P.E. and Morris, K. (2010) 'Low-intensity exercise enhances expression of markers of alternative activation in circulating leukocytes: roles of PPARγ and Th2 cytokines', Atherosclerosis, 212(2), pp.668-673.en_US
dc.identifier.issn0021-9150
dc.identifier.urihttp://hdl.handle.net/10369/5657
dc.description.abstractObjective: Pharmacological activation of the nuclear receptor PPAR is linked to numerous beneficial effects in the contexts of inflammation, lipid homeostasis, Type-2 Diabetes (T2D) and atherosclerosis. These beneficial effects include priming of circulating monocytes for differentiation towards an 'alternative' anti-inflammatory M2 macrophage phenotype. As we have recently shown that participation in low-intensity exercise increases PPAR expression and activity in leukocytes from previously sedentary individuals, we aimed to elucidate whether low-intensity exercise elicited a pattern of gene expression similar to that reported for M2 monocyte-macrophage differentiation. Methods: 17 sedentary individuals undertook an 8-week low-intensity exercise programme (walking 10,000 steps/day, three times/week). Changes in expression of PPARs and the PPAR co-activators PGC- 1 and PGC-1 ; Th2 (IL-4; IL-10) and Th1 (IL-6) cytokines; and markers for the M2 (AMAC1, CD14, MR, IL-4) and the 'classical' pro-inflammatory M1 (MCP-1, TNF , IL-6) phenotypes, were determined using RT-PCR (to assess leukocyte mRNA expression) and ELISA (to assess plasma cytokine levels). Results: Exercise was associated with upregulation of M2 markers, PGC-1 and PGC-1 , and with downregulation of M1 markers. Moreover, plasma levels of Th2 cytokines increased after exercise, while those of Th1 cytokines decreased. However, other PPARs (PPAR ; PPAR / ) did not undergo marked exerciseinduced activation or upregulation. Thus, participation in low-intensity exercise may prime monocytes for differentiation towards an M2 macrophage phenotype via PPAR /PGC-1 / . Conclusion: Given the similarities between these effects and pharmacologically induced M2 polarisation, we propose that exercise-induced PPAR /PGC-1 / -mediated M2 polarisation may constitute a novel anti-inflammatory benefit of low-intensity exercise. © 2010 Elsevier Ireland Ltd. All rights reserved.
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesAtherosclerosis
dc.subjectexerciseen_US
dc.subjectmonocyte polarisationen_US
dc.subjectanti-inflammatoryen_US
dc.subjectanti-atherogenicen_US
dc.subjectPPAR-yen_US
dc.titleLow-intensity exercise enhances expression of markers of alternative activation in circulating leukocytes: Roles of PPARy and Th2 cytokinesen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.atherosclerosis.2010.07.002


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