AMPK agonists are exercise mimetics via PGC1alpha in monocytic cells
Al Mawa'ali, Ruqiya Mohammed Hammed
Cardiff Metropolitan University
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Introduction: There are evidence shows that exercise can help to maintain healthy weight and promote healthy growth and development as well as it maintains the body's energy balance. Also, exercise reduces development's risk of many diseases like stroke, high blood pressure, heart disease and type two diabetes. This occur via the link of exercise and gene expression throw the role of AMPK/ATP ratio which activate AMPK to phosphorylate PGC1α and activate PPARy signaling system.it is noteworthy that exercise mimetics have the same affect of exercise itself on this pathway. Methods: THP-1 cells were cultured in RPMI and treated with oligomycin for 15 minutes. Proteins were extracted from them later to do Co-Immunoprecipitation, electrophoresis and western blotting to test the AMPK activation. Results: AMPK was activated in THP-1 monocytes by oligomycin. This treatment results in phosphorylation of PGCIα and upregulation of PPARy as band of AMPK (activated) was exist. Conclusion: the affect of oligomycin on activating AMP that phosphorylate PGC1a, and thus activating PPARy signaling, make it a fascinating target for pharmacological mediation in treating diseases associated with sedentary life style.
BSc (Hons) Biomedical Science
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