The role of conjugated linoleic acid in regulating the inflammatory response in human monocytic cells
Lin, Thet Thet
Cardiff Metropolitan University
MetadataShow full item record
Type 2 Diabetes (T2D) is an important disorder that represents one of the most serious healthcare challenges facing the world. An estimated 293 million people worldwide are expected to have the condition by the year 2020. T2D is associated with increased cardiovascular and atherosclerotic risk, which are themselves due to increased levels of inflammation present in the disease. Inflammation in T2D is associated with the production of Advanced Glycation End-Products (AGEs), a result of chronically increased blood glucose levels. Clinical studies have demonstrated that the majority of diabetics suffer from cardiovascular disease, which accounts for 80% of all diabetic deaths. The peroxisome proliferator receptors (PPARs), an important group of nuclear receptors are members of the steroid and thyroid hormone receptor family of transcription factors and are increasingly seen as targets in the management of T2D. The dietary polyunsaturated fatty acid, Conjugated Linoleic Acid (CLA) has received considerable attention due to its anti-diabetes and anti-inflammatory effects. CLA has binding affinity for both PPAR-α and -γ and may influence both the cardiovascular risk associated with T2D and also the risk of developingT2D. This study investigated the role of CLA in regulating the inflammatory response of human monocytes. Inflammation was induced using glycated-BSA (Gly-BSA), an Amadori product that consists of 95% protein with 1-5 moles of hexose (as fructosamine) per mole albumin. Gly-BSA acts in a similar way to AGEs to activate monocytes/macrophages by binding to a surface receptor called RAGE. Stimulation of RAGE by gly-BSA leads to activation of a signal transduction pathway, which results in subsequent production of inflammatory cytokines, including Tumour Necrosis Factor -α. Treatment with CLA at a concentration of 60µM significantly reduced (p<0.001, ANOVA) gly-BSA stimulated production of Tumour Necrosis Factor-α, Interleukin-lß, Prostaglandin E2 and Cyclooxygenase-2 by the human monocytic cell-line MM6. CLA was found to modulate the activity of both the nuclear transcription factor NF-KB and RAGE in these cells. Treating MM6 cells with antisense oligonucleotide to PPAR-α and also to PPAR-γ revealed that the modulatory effects of CLA were mediated through PPAR-α but not PPAR-γ. In contrast, CLA treatment did not reduce gly-BSA stimulated Tumour Necrosis Factor-α and RAGE expression in another human monocytic/macrophage cell line, differentiated THP-1 macrophages and also in human-peripheral blood monocyte derived macrophages. The increase in RAGE expression and TNF-α secretion by CLA in these macrophages appeared to be mediated through both PPAR-γ and PPAR-α mediated mechanisms. Taken together these findings indicate CLA can interfere with the inflammatory processes of monocytic cell lines; and that both down-regulation and up-regulation of inflammatory processes by CLA are cell-specific, and mediated via both PPAR-γ and PPAR-α.
Showing items related by title, author, subject and abstract.
An investigation into motility characteristics in THP-1 cells - relevance for type 2 diabetes mellitus Wheat, Richard (Cardiff Metropolitan University, 2011)In recent times, it has become established that common obesity linked Type 2 Diabetes Mellitus (T2DM) is a disease with an underlying chronic systemic inflammatory component, a central feature of which is the infiltration ...
Alhadidi, Younis (Cardiff Metropolitan University, 2011)Objective: Conjugated linoleic acid (CLA) is a group of naturally occurring fatty acids found in food of dairy products in ruminated animals. CLA found to have beneficial effects in animal models as it has anti-atherosclerotic ...
The effects on markers of cellular stress and RAGE splicing in cellular models and in subjects receiving dietary CLA supplementation Mainwaring, Lowri (Cardiff Metropolitan University, 2012)The dietary lipid Conjugated Linoleic Acid (CLA) has been the subject of a large body of research in relation to its anti-inflammatory and immunoregulatory role in many cell types and animal models. This study aimed to ...