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A methanolic extract of Trigonella foenum-graecum (Fenugreek) seeds regulates markers of macrophage polarization

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Author
Hassan, Nurudeen
Withycombe, Cathryn
Ahluwalia, Maninder
Thomas, Andrew
Morris, Keith
Date
2015
Type
Article
ISSN
2160-3855
Metadata
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Abstract
Background: Macrophages are key cellular mediators in diabetes-related inflammation. Molecular cues such as cytokines found in the tissue microenvironment regulates the polarization of macrophages into an M1 (pro-inflammatory) or M2 (immunoregulatory) phenotype. Recent evidence suggests that M1 macrophages in diabetic patients may contribute to the complications associated with the disease such as atherosclerosis. Trigonella foenum-graecum(Tfg: fenugreek) seeds have been used in traditional medicinein Asia, Africa and the Middle-East for their alleged anti-diabetic properties. Objective: To identify the molecular mechanism(s) through which Tfgseeds exert their effects, we investigated the role of a crude methanolicextract of Tfgseeds(FME) on macrophage polarization in vitro. Method: THP-1 macrophages(Mφ)were treated withgBSAin the presence/absence of FME and the release and expression of M1 and M2 markers/cytokines were analysed. The role of FME onNF-κBactivity was also explored using transfected HEK-293Tcells. Results: This study found that the FME significantly (P<0.05) decreased gBSA-induced secretion of M1 cytokines (TNF-α, IL-1β, IL-6 and IL-8) in THP-1 Mφcells. In the presence of gBSA, FME also significantly increased the gene expression of the M2 marker Dectin-1, but had no effect on IL-10, IL-1Ra. FME also significantly decreased TNF-α induced NF-kB reporter activity. Conclusion:These results suggest that FME can regulate the expression of M1 and M2 markers in THP-1 Mφcells. This may be potentially through the modulation of NF-kB activity. Further work should be carried out to identify precise mechanism(s)involved in the effects of FMEand Tfg seeds in diabetes and other conditions.
Journal/conference proceeding
Journal of Functional Foods in Health and Disease
Citation
Hassan, N., Withycombe, C., Ahluwalia, M., Thomas, A. and Morris, K. (2015) 'A methanolic extract of Trigonella foenum-graecum (fenugreek) seeds regulates markers of macrophage polarization', Journal of Functional Foods in Health and Disease, 5 (12), pp. 417-426
URI
http://www.ffhdj.com/index.php/ffhd/article/view/216/441
http://hdl.handle.net/10369/7666
Rights
This article has been published in Journal of Functional Foods in Health and Disease (an open access journal), available at: http://www.ffhdj.com/index.php/ffhd/article/view/216/441
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  • Cardiovascular Health and Ageing [100]

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