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dc.contributor.authorAdams, Rachel
dc.contributor.authorAl-Mosawi, Ameena
dc.contributor.authorBérubé, Kelly
dc.contributor.authorJones, Tim
dc.contributor.authorHiggins, Timothy
dc.contributor.authorEvans, Shelley-Ann
dc.date.accessioned2016-02-19T17:33:29Z
dc.date.available2016-02-19T17:33:29Z
dc.date.issued2014
dc.identifier.citationAdams, R.A., Al-Mosawi, A., Berube, K., Jones, T., Higgins, T. and Evans, S-A (2014) 'Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles', Biorheology, 51, pp. 329-338en_US
dc.identifier.issn0006-355X
dc.identifier.urihttp://hdl.handle.net/10369/7717
dc.descriptionThis article was published in Biorheology in 2014. The final published version is freely available at: http://dx.doi.org/10.3233/BIR-14027.en_US
dc.description.abstractBACKGROUND: Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood. OBJECTIVE: The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leukocyte flow properties by measurement of polymorphonucleocyte (PMN) and monocyte actin polymerisation. METHODS: Rats were exposed to particulate air pollution by intratracheal instillation of PM10. Blood was collected from test and control animals at 3 days (n=10) and 6 weeks (n=10) after dust instillation. Partial differential leukocyte counts were performed. The intracellular F-actin content of blood PMNs and monocytes was determined by staining with FITC-phalloidin and flow cytometric determination of mean florescence intensity (MFI). RESULTS: There were no significant changes in PMN MFI (p=0.369, ANOVA) or cell counts (p=0.753, ANOVA). There was a significant increase in monocyte MFI (p=0.004, ANOVA) and a decrease in monocyte cell count (p=0.003, ANOVA) in instilled rats. CONCLUSIONS: Intratracheal instillation of air pollution particles resulted in an increase in blood monocyte actin polymerisation, which may cause trapping of monocytes. This could be a mechanism by which exposure to air pollution increases the risk of cardiovascular disease.en_US
dc.language.isoenen_US
dc.publisherIOS Press Content Libraryen_US
dc.relation.ispartofseriesBiorheology;
dc.rightsNon-commercial use only
dc.subjectparticlesen_US
dc.subjectleukocyteen_US
dc.subjectneutrophilen_US
dc.subjectcytoskeletonen_US
dc.subjectcardiovascular diseaseen_US
dc.titleIncreased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particlesen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.3233/BIR-14027
dc.date.dateAccepted2014-09-27


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