Inhibition of biofilms of Pseudomonas aeruginosa by Medihoney in vitro
Mark Allen Healthcare
MetadataDangos cofnod eitem llawn
OBJECTIVE: Pseudomonas aeruginosa has been linked to chronic wound infections, where its ability to form biofilms and to tolerate antimicrobial agents helps to facilitate its persistence. This study aimed to investigate the susceptibility of biofilms of Pseudomonas aeruginosa to Medihoney in vitro. METHOD: Biofilms were cultivated in microtitre plates with and without a range of concentrations of Medihoney, and effects on biofilm were monitored by optical density (at 650nm), biomass (by staining with crystal violet), metabolic activity (using an esterase assay) and viability (by determining total cell counts). Structural effects on established biofilms were examined by scanning electron microscopy and epifluorescence following staining by LIVE/DEAD® BacLight, which also showed effects on vitality. RESULTS: The lowest concentration of Medihoney found to prevent biofilm formation was 17%(w/v), whereas on average 35.5%(w/v) of Medihoney was required to inhibit established biofilms. Susceptibility did not vary with length of biofilm establishment between 24 and 72 hours. Extensive structural changes in established biofilms were seen in the sample with less than or equal to 30%(w/v) Medihoney using scanning electron microscopy and loss of viability was found in test samples with less than or equal to 20%(w/v) Medihoney concentration using fluorescent staining, together with loss of biofilm structure. CONCLUSION: Using a range of methods to evaluate biofilm integrity, this study demonstrates that Medihoney inhibits Pseudomonas aeruginosa biofilms in vitro at concentrations that are attainable in clinical use. Whether Medihoney has the potential to disrupt Pseudomonas aeruginosa biofilms in cutaneous wounds must now be tested in patients.
Journal of Wound Care
Cooper, R., Jenkins, L. and Hooper, S., (2014) 'Inhibition of biofilms of Pseudomonas aeruginosa by Medihoney in vitro', Journal of Wound Care, 23(3), pp.93-6.
Dynodwr Gwrthrych Digidol (DOI)http://dx.doi.org/10.12968/jowc.2014.23.3.93
This article was published in Journal of Wound Care on 26th March 2014 (online), available at http://dx.doi.org/10.12968/jowc.2014.23.3.93
This study was sponsored by Derma Sciences Inc, NJ. An unrestricted grant was provided and the sponsors were not involved in the design of the experiments or the preparation of this manuscript.
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Okhiria, Olusola Adeola (University of WalesCardiff School of Health Sciences, 2010)Biofilms have long been implicated in persistent infections and have recently been associated with chronic wounds. The role of bacteria in wounds is not yet fully understood and their ability to form biofilm is yet to be ...
Alhinai, Asma (University of Wales Institute Cardiff, 2012)Wound healing is a complex process with many probable factors that can delay healing; wound infection, especially biofilm infection, is one of these delaying factors. The wound is colonized by bacteria and the bacteria ...
Manuka honey treatment of biofilms of Pseudomonas aeruginosa results in the emergence of isolates with increased honey resistance Camplin, A. L.; Maddocks, Sarah (BioMed Central, 2014)Background Medical grade manuka honeys are well known to be efficacious against Pseudomonas aeruginosa being bactericidal and inhibiting the development of biofilms; moreover manuka honey effectively kills P. aeruginosa ...