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dc.contributor.authorRoberts, Aled Edward Lloyd
dc.contributor.authorMaddocks, Sarah
dc.contributor.authorCooper, Rose
dc.date.accessioned2016-02-26T10:38:35Z
dc.date.available2016-02-26T10:38:35Z
dc.date.issued2014
dc.identifier.citationRoberts, A.E.L., Maddocks, S.E. and Cooper, R.A. (2014) 'Manuka honey reduces the motility of Pseudomonas aeruginosa by suppression of flagella-associated genes', Journal of Antimicrobial Chemotherapy.en_US
dc.identifier.issn0305-7453
dc.identifier.urihttp://hdl.handle.net/10369/7727
dc.descriptionThis article was published in Journal of Antimicrobial Chemotherapy on 16th November 2014, available at http://dx.doi.org/10.1093/jac/dku448en_US
dc.description.abstractObjectives Manuka honey is a broad-spectrum antimicrobial agent that seems to affect different bacteria in many different ways. It has been shown to be bactericidal against Pseudomonas aeruginosa by destabilizing the cell wall, but we aimed to investigate whether there were further intracellular target sites. Methods In this study inhibitory effects of manuka honey on P. aeruginosa were investigated using hydrophobicity assays, two-dimensional electrophoresis, quantitative RT–PCR, transmission electron microscopy and motility assays. Results Exposure of P. aeruginosa to manuka honey reduced both swarming and swimming motility. Moreover, this was a consequence of de-flagellation of the bacterial cell, which was correlated with decreased expression of the major structural flagellin protein, FliC, and concurrent suppression of flagellin-associated genes, including fliA, fliC, flhF, fleN, fleQ and fleR. The differential expression of the flagellar regulon in the presence of manuka honey was mapped schematically. Flagella are integral to bacterial adhesion, the initiation of infection and biofilm formation, and swarming has been associated with increased virulence. Conclusions By limiting motility in vitro, we infer that manuka honey impacts on the virulence of P. aeruginosa. This deduction must now be tested in vivo.en_US
dc.language.isoenen_US
dc.publisherOxford Journalsen_US
dc.relation.ispartofseriesJournal of Antimicrobial Chemotherapy
dc.subjecthydrophobicityen_US
dc.subjectswimmingen_US
dc.subjectswarmingen_US
dc.subjectvirulenceen_US
dc.subjectwound infectionen_US
dc.titleManuka honey reduces the motility of Pseudomonas aeruginosa by suppression of flagella-associated genesen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dku448
dc.date.dateAccepted2014-10-14


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