Quantitative high-throughput gene expression profiling of human striatal development to screen stem cell–derived medium spiny neurons
Author
Straccia, Marco
Barriga, Gerardo Garcia-Diaz
Sanders, Phil
Bombau, Georgina
Carrere, Jordi
Mairal, Pedro Belio
Vinh, Ngoc-Nga
Yung, Sun
Kelly, Claire
Svendsen, Clive N.
Kemp, Paul J.
Arjomand, Jamshid
Schoenfeld, Ryan C.
Alberch, Jordi
Allen, Nicholas D.
Rosser, Anne E.
Canals, Josep M.
Date
2015Type
Article
Publisher
Nature.com
Metadata
Show full item recordAbstract
A systematic characterization of the spatio-temporal gene expression during human neurodevelopment is essential to understand brain function in both physiological and pathological conditions. In recent years, stem cell technology has provided an in vitro tool to recapitulate human development, permitting also the generation of human models for many diseases. The correct differentiation of human pluripotent stem cell (hPSC) into specific cell types should be evaluated by comparison with specific cells/tissue profiles from the equivalent adult in vivo organ. Here, we define by a quantitative high-throughput gene expression analysis the subset of specific genes of the whole ganglionic eminence (WGE) and adult human striatum. Our results demonstrate that not only the number of specific genes is crucial but also their relative expression levels between brain areas. We next used these gene profiles to characterize the differentiation of hPSCs. Our findings demonstrate a temporal progression of gene expression during striatal differentiation of hPSCs from a WGE toward an adult striatum identity. Present results establish a gene expression profile to qualitatively and quantitatively evaluate the telencephalic hPSC-derived progenitors eventually used for transplantation and mature striatal neurons for disease modeling and drug-screening.
Journal/conference proceeding
Molecular therapy. Methods & Clinical Development
Citation
Straccia, M., Barriga, G.G.D., Sanders, P., Bombau, G., Carrere, J., Mairal, P.B., Vinh, N.N., Yung, S., Kelly, C.M., Svendsen, C.N., Kemp, P.J., Arjomand, J., Schoenfeld, R.C., Alberch, J., Allen, N.D., Rosser, A.E. & Canals, J.M. (2015) 'Quantitative high-throughput gene expression profiling of human striatal development to screen stem cell–derived medium spiny neurons', Molecular Therapy. Methods & Clinical Development, 2, p.15030.
Description
This article was published in Molecular therapy. Methods & Clinical Development on 16th September 2015 (online), available at http://dx.doi.org/10.1038/mtm.2015.30
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