Proof of concept: containment systems that prevent freeze-drier contamination when lyophilising Escherichia coli (JM 109)
Taylor and Francis
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This study describes the use of containment systems to prevent escape of microorganisms during lyophilization, thereby avoiding contamination of freeze-drying equipment. Cultures of Escherichia coli (JM 109) of an approximate cell concentration of 109 cfu/mL were suspended in 0.9% saline, aseptically dispensed into vials, double-wrapped in either medical-grade paper or Tyvek sterilization pouches and freeze-dried. An intentional collapse phenomenon was observed during the freeze-drying process, ejecting debris and aerosols from the vials, thus representing a worst-case challenge for containment. Following freeze drying, the layers of the pouches were tested for microbial contamination using 3 M Clean-Trace sur- face ATP analyzer swabs, surface swabs, and tryptone soya agar contact plates. The paper and Tyvek pouches were able to contain a maximum cell concentration of 1 x 106 cfu/mL of E. coli recov- ered from ejected debris. Some penetration through the ﬁrst paper pouch layer was observed (although not Tyvek); however, this was successfully retained by the second, outer layer preventing contami- nation of the lyophilization apparatus and laboratory environment.
Cherry, C.L.A., Cooper, R., Millward, H. and Landon, J. (2015) 'Proof of concept: containment systems that prevent freeze-dryer contamination when lyophilizing Escherichia coli (JM 109)', Drying Technology, 33(4), pp.466-470.
This article was published in Drying Technology on 7 January 2015 (online), available at http://dx.doi.org/10.1080/07373937.2014.963204
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