Proof of concept: containment systems that prevent freeze-drier contamination when lyophilising Escherichia coli (JM 109)
Taylor and Francis
MetadataDangos cofnod eitem llawn
This study describes the use of containment systems to prevent escape of microorganisms during lyophilization, thereby avoiding contamination of freeze-drying equipment. Cultures of Escherichia coli (JM 109) of an approximate cell concentration of 109 cfu/mL were suspended in 0.9% saline, aseptically dispensed into vials, double-wrapped in either medical-grade paper or Tyvek sterilization pouches and freeze-dried. An intentional collapse phenomenon was observed during the freeze-drying process, ejecting debris and aerosols from the vials, thus representing a worst-case challenge for containment. Following freeze drying, the layers of the pouches were tested for microbial contamination using 3 M Clean-Trace sur- face ATP analyzer swabs, surface swabs, and tryptone soya agar contact plates. The paper and Tyvek pouches were able to contain a maximum cell concentration of 1 x 106 cfu/mL of E. coli recov- ered from ejected debris. Some penetration through the ﬁrst paper pouch layer was observed (although not Tyvek); however, this was successfully retained by the second, outer layer preventing contami- nation of the lyophilization apparatus and laboratory environment.
Cherry, C.L.A., Cooper, R., Millward, H. and Landon, J. (2015) 'Proof of concept: containment systems that prevent freeze-dryer contamination when lyophilizing Escherichia coli (JM 109)', Drying Technology, 33(4), pp.466-470.
Dynodwr Gwrthrych Digidol (DOI)http://dx.doi.org/10.1080/07373937.2014.963204
This article was published in Drying Technology on 7 January 2015 (online), available at http://dx.doi.org/10.1080/07373937.2014.963204
Knowledge Economy Skills Scholarship (KESS)
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Cherry, Christopher Lee Albert (Cardiff Metropolitan University, 2013)This thesis investigates the novel use of paper and Tyvek sterilisation pouches as containment systems to perform various applications of freeze-drying. Their effect on the freeze-drying of a pharmaceutical protein, mass ...
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