Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array
Welton, Joanne Louise
Webber, Jason Paul
Spray, Lisa Kate
Carton, David Gil
Falcon-Perez, Juan Manuel
Walton, Sean Peter
Mason, Malcolm David
MetadataShow full item record
Proteomics analysis of biofluid-derived vesicles holds enormous potential for discovering non-invasive disease markers. Obtaining vesicles of sufficient quality and quantity for profiling studies has, however, been a major problem, as samples are often replete with co-isolated material that can interfere with the identification of genuine low abundance, vesicle components. Here, we used a combination of ultracentrifugation and size-exclusion chromatography to isolate and analyse vesicles of plasma or urine origin. We describe a sample-handling workflow that gives reproducible, quality vesicle isolations sufficient for subsequent protein profiling. Using a semi-quantitative aptamer-based protein array, we identified around 1,000 proteins, of which almost 400 were present at comparable quantities in plasma versus urine vesicles. Significant differences were, however, apparent with elements like HSP90, integrin αVβ5 and Contactin-1 more prevalent in urinary vesicles, while hepatocyte growth factor activator, prostate-specific antigen–antichymotrypsin complex and many others were more abundant in plasma vesicles. This was also applied to a small set of specimens collected from men with metastatic prostate cancer, highlighting several proteins with the potential to indicate treatment refractory disease. The study provides a practical platform for furthering protein profiling of vesicles in prostate cancer, and, hopefully, many other disease scenarios.
Journal of Extracellular Vesicles
Welton, J.L., Brennan, P., Gurney, M., Webber, J.P., Spray, L.K., Carton, D.G., Falcon-Perez, J.M., Walton, S.P., Mason, M.D., Tabi, Z. and Clayton, A. (2016) 'Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array', Journal of Extracellular Vesicles, 5, 31209
This article was published in Journal of Extracellular Vesicles on 29 June 2016 (online), available at http://dx.doi.org/10.3402/jev.v5.31209
RightsCreative Commons Attribution Non-Commercial 3.0 Unported License
- Un-themed 
Except where otherwise noted, this item's license is described as Creative Commons Attribution Non-Commercial 3.0 Unported License
Showing items related by title, author, subject and abstract.
Welton, Joanne Louise; Webber, Jason Paul; Botos, Laur-Alexandru; Jones, Michael; Clayton, Aled (Co-action Publishing, 2015)Proteomic studies of circulating vesicles are hampered by difficulties in purifying vesicles from plasma and serum. Isolations are contaminated with high-abundance blood proteins that may mask genuine vesicular-associated ...
Ambler, Nicola (University of Wales Institute Cardiff, 2012)Introduction: Prostate cancer is the second most commonly diagnosed cancer in men worldwide. Currently the biomarker used to diagnose and manage prostate cancer is Prostate Specific Antigen (PSA). Due to its associated ...
Cerebrospinal fluid extracellular vesicle enrichment for protein biomarker discovery in neurological disease; multiple sclerosis Welton, Joanne Louise; Loveless, Samantha; Stone, Timothy; von Ruhland, Chris; Robertson, Neil; Clayton, Aled (Taylor & Francis, 2017-09-03)The discovery of disease biomarkers, along with the use of “liquid biopsies” as a minimally invasive source of biomarkers, continues to be of great interest. In inflammatory diseases of the central nervous system (CNS), ...