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dc.contributor.authorWelton, Joanne Louise
dc.contributor.authorBrennan, Paul
dc.contributor.authorGurney, Mark
dc.contributor.authorWebber, Jason Paul
dc.contributor.authorSpray, Lisa Kate
dc.contributor.authorCarton, David Gil
dc.contributor.authorFalcon-Perez, Juan Manuel
dc.contributor.authorWalton, Sean Peter
dc.contributor.authorMason, Malcolm David
dc.contributor.authorTabi, Zsuzsanna
dc.contributor.authorClayton, Aled
dc.date.accessioned2016-06-30T15:53:29Z
dc.date.available2016-06-30T15:53:29Z
dc.date.issued2016-06-29
dc.identifier.citationWelton, J.L., Brennan, P., Gurney, M., Webber, J.P., Spray, L.K., Carton, D.G., Falcon-Perez, J.M., Walton, S.P., Mason, M.D., Tabi, Z. and Clayton, A. (2016) 'Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array', Journal of Extracellular Vesicles, 5, 31209en_US
dc.identifier.issn2001-3078
dc.identifier.urihttp://hdl.handle.net/10369/7950
dc.descriptionThis article was published in Journal of Extracellular Vesicles on 29 June 2016 (online), available open access at http://dx.doi.org/10.3402/jev.v5.31209
dc.description.abstractProteomics analysis of biofluid-derived vesicles holds enormous potential for discovering non-invasive disease markers. Obtaining vesicles of sufficient quality and quantity for profiling studies has, however, been a major problem, as samples are often replete with co-isolated material that can interfere with the identification of genuine low abundance, vesicle components. Here, we used a combination of ultracentrifugation and size-exclusion chromatography to isolate and analyse vesicles of plasma or urine origin. We describe a sample-handling workflow that gives reproducible, quality vesicle isolations sufficient for subsequent protein profiling. Using a semi-quantitative aptamer-based protein array, we identified around 1,000 proteins, of which almost 400 were present at comparable quantities in plasma versus urine vesicles. Significant differences were, however, apparent with elements like HSP90, integrin αVβ5 and Contactin-1 more prevalent in urinary vesicles, while hepatocyte growth factor activator, prostate-specific antigen–antichymotrypsin complex and many others were more abundant in plasma vesicles. This was also applied to a small set of specimens collected from men with metastatic prostate cancer, highlighting several proteins with the potential to indicate treatment refractory disease. The study provides a practical platform for furthering protein profiling of vesicles in prostate cancer, and, hopefully, many other disease scenarios.en_US
dc.language.isoenen_US
dc.publisherCo-Actionen_US
dc.relation.ispartofseriesJournal of Extracellular Vesicles
dc.rightsCreative Commons Attribution Non-Commercial 3.0 Unported License
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.subjectcanceren_US
dc.subjectprostateen_US
dc.subjectproteomicsen_US
dc.subjectvesiclesen_US
dc.subjectplasmaen_US
dc.subjectprotein arrayen_US
dc.subjecturineen_US
dc.titleProteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein arrayen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.3402/jev.v5.31209
dc.date.dateAccepted2016-04-17


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