Detailed characterisation of circulatory nitric oxide and free radical indices—is there evidence for abnormal cardiovascular homeostasis in young women with polycystic ovary syndrome ?
Wiley Online Library
MetadataShow full item record
Abstract OBJECTIVE: To assess circulating biochemical indices of endothelial function and nitro-oxidative stress in women with polycystic ovary syndrome (PCOS). DESIGN: Case-control study. POPULATION: Seventeen women with PCOS and eighteen age- and body mass index-matched healthy volunteers. METHODS: Nitric oxide (NO) metabolite levels were assessed by chemiluminescence. Electron paramagnetic resonance spectroscopy with spin trapping was used to assess oxidative stress ex vivo and in vitro. Antioxidant capacity was measured using oxygen radical absorbance. MAIN OUTCOME MEASURES: Biochemical indices of endothelial function, including NO metabolites, lipid-derived radicals and antioxidant capacity. RESULTS: Plasma NO metabolites were similar in the two groups (nitrite: 257±116 nmol/l [PCOS], 261±135 nmol/l [controls] P=0.93; nitrate: 27±7 μmol/l [PCOS], 26±6 μmol/l [controls] P=0.89). Alkoxyl free radicals (lipid-derived) were detected as the dominant species, but levels were not different between women with PCOS and controls whether measured directly ex vivo (median 7.2 [range 0.17-16.73]e6 arbitrary units [a.u.] and 7.2 [1.7-11.9]e6 a.u., respectively, P=0.57) or when stimulated in vitro to test radical generation capacity (1.23 [0.3-5.62]e7 a.u. and 1.1 [0.48-15.7]e7 a.u. respectively, P=0.71). In regression analysis, visceral fat area was independently associated with in vitro oxidative potential (β=0.6, P=0.002). Total plasma antioxidant capacity (94±30% [PCOS], 79±24% [controls], P=0.09) and plasma hydroperoxides (7.5±4 μmol/l [PCOS], 6.7±5 μmol/l [controls], P=0.21) were not different between groups. However, lipophilic antioxidant capacity was lower in women with PCOS compared with controls (92±32 and 125±48%, respectively, P=0.02). CONCLUSIONS: Young overweight women with PCOS display a reduced lipophilic antioxidant capacity compared with healthy volunteers, but no change in circulating free radicals or nitro-oxidative stress.
BJOG: An International Journal of Obstetrics & Gynaecology;
Willis, G.R., Udiawar, M., Evans, W.D., Blundell, H.L., James, P.E. and Rees, D.A. (2014) 'Detailed characterisation of circulatory nitric oxide and free radical indices—is there evidence for abnormal cardiovascular homeostasis in young women with polycystic ovary syndrome?', BJOG: An International Journal of Obstetrics & Gynaecology, 121(13), pp.1596-1603
This article was published in BJOG: An International Journal of Obstetrics & Gynaecology on 12 May 2014 (online), available at http://dx.doi.org/10.1111/1471-0528.12834
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
The Wales Heart Research Institute
Showing items related by title, author, subject and abstract.
UBC-Nepal expedition: The use of oral antioxidants does not alter cerebrovascular function at sea-level or high-altitude Hansen, Alex B.; Hoiland, Ryland L.; Lewis, Nia C. S.; Tymko, Michael M.; Tremblay, Joshua C.; Stembridge, Mike; Nowak-Fluck, Daniela; Carter, Howard H.; Bailey, Damian M.; Ainslie, Philip (Wiley, 2018-02-10)Hypoxia is associated with an increased systemic and cerebral formation of free radicals and associated reactants that may be linked to impaired cerebral vascular function a neurological sequela. To what extent oral ...
Wilkin, Jonathan; Ashton, Ian; Fielding, Louise; Tatham, Arthur (Springer, 2014)Peanuts are increasingly being used as nibbed ingredients in cereal bars, confectionery and breakfast cereals. However, studies on their oxidative stability in this format are limited. Storage trials to determine the ...
Systemic oxidative–nitrosative–inflammatory stress during acute exercise in hypoxia; implications for microvascular oxygenation and aerobic capacity Woodside, John D.S; Gutowski, Mariusz; Fall, Lewis; James, Philip; McEneny, Jane; Young, Ian S.; Ogoh, Shigehiko; Bailey, Damian M. (Wiley Online Library, 2014-12-01)New Findings •What is the central question of this study? Exercise performance is limited during hypoxia by a critical reduction in cerebral and skeletal tissue oxygenation. To what extent an elevation in systemic ...