Antimicrobial activity of Manuka honey against antibiotic resistant strains of the cell wall free bacteria Ureaplasma parvum and Ureaplasma urealyticum
Beeton, Michael L.
MetadataShow full item record
The susceptibility of the cell-wall free bacterial pathogens Ureaplasma spp. to Manuka honey was examined. The minimum inhibitory concentration (MIC) of Manuka honey for four Ureaplasma urealyticum and four Ureaplasma parvum isolates was determined. Sensitivity to honey was also compared to clinical isolates with resistance to tetracycline, macrolide and fluoroquinolone antibiotics. Finally step-wise resistance training was utilised in an attempt to induce increased tolerance to honey. The MIC was dependent on the initial bacterial load with 7.5% and 18.0% w/v honey required to inhibit U. urealyticum at 1 and 106 colour changing units (CCU), respectively, and 4.8% and 15.3% w/v required to inhibit U. parvum at 1 and 106 CCU, respectively. MIC values were consistently lower for U. parvum compared with U. urealyticum. Antimicrobial activity was seen against tetracycline resistant, erythromycin resistant and ciprofloxacin resistant isolates at 105 CCU. No resistance to honey was observed with fifty consecutive challenges at increasing concentrations of honey. This is the first report of the antimicrobial activity of Manuka honey against a cell-wall free bacterial pathogen. The antimicrobial activity was retained against antibiotic resistant strains and it was not possible to generate resistant mutants.
Letters In Microbiology
Hillitt, K.L., Jenkins, R.E., Spiller, O.B. and Beeton, M.L. (2016) 'Antimicrobial activity of Manuka honey against antibiotic resistant strains of the cell wall free bacteria Ureaplasma parvum and Ureaplasma urealyticum', Letters in Applied Microbiology, 64 (3), pp.198-202
This article was published in Letters in Applied Microbiology on 19 December 2016 (online), available at http://dx.doi.org/doi: 10.1111/lam.12707
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
Showing items related by title, author, subject and abstract.
Roberts, Aled Edward Lloyd; Brown, Helen Louise; Jenkins, Rowena (Dove press, 2015-10-29)Antimicrobial resistance (AMR) is an increasing clinical problem precipitated by the inappropriate use of antibiotics in the later parts of the 20th Century. This problem, coupled with the lack of novel therapeutics in ...
Manuka honey treatment of biofilms of Pseudomonas aeruginosa results in the emergence of isolates with increased honey resistance Camplin, A. L.; Maddocks, Sarah (BioMed Central, 2014)Background Medical grade manuka honeys are well known to be efficacious against Pseudomonas aeruginosa being bactericidal and inhibiting the development of biofilms; moreover manuka honey effectively kills P. aeruginosa ...
Henriques, Ana (University of Wales, 2006)Honey is an old remedy recently rediscovered as a possible alternative to modern antibiotics in wound management but its mode of action is not fully understood. The antibacterial activity of honey can be divided into ...