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dc.contributor.authorHillitt, K.L.
dc.contributor.authorJenkins, Rowena
dc.contributor.authorSpiller, O.B.
dc.contributor.authorBeeton, Michael L.
dc.date.accessioned2017-01-24T10:21:40Z
dc.date.available2017-01-24T10:21:40Z
dc.date.issued2016-12-19
dc.identifier.citationHillitt, K.L., Jenkins, R.E., Spiller, O.B. and Beeton, M.L. (2016) 'Antimicrobial activity of Manuka honey against antibiotic resistant strains of the cell wall free bacteria Ureaplasma parvum and Ureaplasma urealyticum', Letters in Applied Microbiology, 64 (3), pp.198-202en_US
dc.identifier.urihttp://hdl.handle.net/10369/8313
dc.descriptionThis article was published in Letters in Applied Microbiology on 19 December 2016 (online), available at http://dx.doi.org/doi: 10.1111/lam.12707en_US
dc.description.abstractThe susceptibility of the cell-wall free bacterial pathogens Ureaplasma spp. to Manuka honey was examined. The minimum inhibitory concentration (MIC) of Manuka honey for four Ureaplasma urealyticum and four Ureaplasma parvum isolates was determined. Sensitivity to honey was also compared to clinical isolates with resistance to tetracycline, macrolide and fluoroquinolone antibiotics. Finally step-wise resistance training was utilised in an attempt to induce increased tolerance to honey. The MIC was dependent on the initial bacterial load with 7.5% and 18.0% w/v honey required to inhibit U. urealyticum at 1 and 106 colour changing units (CCU), respectively, and 4.8% and 15.3% w/v required to inhibit U. parvum at 1 and 106 CCU, respectively. MIC values were consistently lower for U. parvum compared with U. urealyticum. Antimicrobial activity was seen against tetracycline resistant, erythromycin resistant and ciprofloxacin resistant isolates at 105 CCU. No resistance to honey was observed with fifty consecutive challenges at increasing concentrations of honey. This is the first report of the antimicrobial activity of Manuka honey against a cell-wall free bacterial pathogen. The antimicrobial activity was retained against antibiotic resistant strains and it was not possible to generate resistant mutants.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofseriesLetters In Microbiology
dc.rightsNon-Commercial
dc.subjectantimicrobials
dc.subjectmicrobial structure
dc.subjectinfection
dc.subjectmicrobial physiology
dc.subjectresistance
dc.titleAntimicrobial activity of Manuka honey against antibiotic resistant strains of the cell wall free bacteria Ureaplasma parvum and Ureaplasma urealyticumen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1111/lam.12707
dcterms.dateAccepted2016-12-13
rioxxterms.funderCardiff Metropolitan Universityen_US
rioxxterms.identifier.projectCardiff Metropolian (Internal)en_US
rioxxterms.versionAMen_US
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_US
rioxxterms.licenseref.startdate2017-01-24
rioxxterms.freetoread.startdate2017-12-19
rioxxterms.funder.project37baf166-7129-4cd4-b6a1-507454d1372een_US


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