Binding of two bacterial biofilms to dialkyl carbamoyl chloride (DACC)-coated dressings in vitro

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Author
Cooper, Rose
Jenkins, Leighton
Date
2016-02-15Acceptance date
2016
Type
Article
Publisher
MA Healthcare
ISSN
0969-0700
Metadata
Show full item recordAbstract
Objective:
To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing in vitro.
Method:
Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm.
Results:
Pseudomonas aeruginosa biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples.
Conclusion:
Biofilms of two different test bacteria bound to dressings in vitro with the DACC-coating on the dressings enhancing the binding of MRSA biofilm.
Declaration of interest:
This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.
Journal/conference proceeding
Journal of Wound Care;
Citation
Cooper, R. and Jenkins, L. (2016) 'Binding of two bacterial biofilms to dialkyl carbamoyl chloride (DACC)-coated dressings in vitro', Journal of Wound Care, 25(2), pp.76-82
Description
This article was published in Journal of Wound Care on 15 February 2016 (online), available at http://dx.doi.org/10.12968/jowc.2016.25.2.76
Sponsorship
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
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