Binding of two bacterial biofilms to dialkyl carbamoyl chloride (DACC)-coated dressings in vitro
MetadataShow full item record
Objective: To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing in vitro. Method: Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm. Results: Pseudomonas aeruginosa biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples. Conclusion: Biofilms of two different test bacteria bound to dressings in vitro with the DACC-coating on the dressings enhancing the binding of MRSA biofilm. Declaration of interest: This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.
Journal of Wound Care;
Cooper, R. and Jenkins, L. (2016) 'Binding of two bacterial biofilms to dialkyl carbamoyl chloride (DACC)-coated dressings in vitro', Journal of Wound Care, 25(2), pp.76-82
This article was published in Journal of Wound Care on 15 February 2016 (online), available at http://dx.doi.org/10.12968/jowc.2016.25.2.76
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
Showing items related by title, author, subject and abstract.
Okhiria, Olusola Adeola (University of WalesCardiff School of Health Sciences, 2010)Biofilms have long been implicated in persistent infections and have recently been associated with chronic wounds. The role of bacteria in wounds is not yet fully understood and their ability to form biofilm is yet to be ...
A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings Duckworth, Peter F.; Rowlands, Richard S.; Barbour, Michele E.; Maddocks, Sarah (Elsevier, 2018-07-19)Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial ...
Electrospun zein/PCL fibrous matrices release tetracycline in a controlled manner, killing Staphylococcus aureus both in biofilms and ex vivo on pig skin, and are compatible with human skin cells Alhusein, Nour; Blagbrough, Ian S.; Beeton, Michael L.; Bolhuis, A.; De Bank, P.A. (Springer / American Association of Pharmaceutical Scientists, 2015)Purpose To investigate the destruction of clinically-relevant bacteria within biofilms via the sustained release of the antibiotic tetracycline from zein-based electrospun polymeric fibrous matrices and to demonstrate ...