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dc.contributor.authorLueangsakulthai, J.
dc.contributor.authorJangpromma, N.
dc.contributor.authorTemsiripong, T.
dc.contributor.authorMckendrick, J.E.
dc.contributor.authorKhunkitti, W.
dc.contributor.authorMaddocks, Sarah
dc.contributor.authorKlaynongsruang, S.
dc.date.accessioned2017-07-31T11:22:41Z
dc.date.available2017-07-31T11:22:41Z
dc.date.issued2017-07-16
dc.identifier.citationLueangsakulthai, J., Jangpromma, N., Temsiripong, T., McKendrick, J.E., Khunkitti, W., Maddocks, S.E. and Klaynongsruang, S. (2017) 'A novel antibacterial peptide derived from Crocodylus siamensis hemoglobin hydrolysate induces membrane permeabilisation causing iron dysregulation, oxidative stress and bacterial death', Journal of Applied Microbiologyen_US
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672 (ESSN)
dc.identifier.urihttp://hdl.handle.net/10369/8671
dc.descriptionThis article was published in Journal of Applied Microbiology on 16 July 2017 (online), available at http://dx.doi.org/10.1111/jam.13539en_US
dc.description.abstractAbstract AIMS: A novel antibacterial peptide from Crocodylus siamensis hemoglobin hydrolysate (CHHs) was characterised for antimicrobial activity. METHODS AND RESULTS: CHHs was hydrolysed for 2 h (2h-CHH), 4 h (4h-CHH), 6 h (6h-CHH) and 8 h (8h-CHH). 8h-CHH showed antibacterial activity against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa at concentrations of 20, 20, 20 and 10 mg ml-1 (w/v), respectively. Fluorescent microscopy revealed that 8h-CHH had bactericidal activity against E. coli and P. aeruginosa. β-galactosidase assay supported by RT-qPCR demonstrated that 8h-CHH resulted in differential expression of genes involved in iron homeostasis (ftnA and bfd) and oxidative stress (sodA, soxR and oxyR). Siderophore assay indicated that 8h-CHH also impaired siderophore production with diminished expression of pvdF. This pattern of gene expression suggests that 8h-CHH triggers the release of free ferric ions in the cytoplasm. However, decreased expression of genes associated with the SOS response (recA and lexA) in combination with neutral comet revealed that no DNA damage was caused by 8h-CHH. Membrane permeabilisation assay indicated that 8h-CHH caused membrane leakage thought to mediate the antibacterial and iron-stress responses observed, due to loss of regulated iron transport. The novel active peptide from 8h-CHH was determined as QAIIHNEKVQAHGKKVL (QL17), with 41% hydrophobicity and +2 net charge. CONCLUSIONS: The QAIIHNEKVQAHGKKVL fragment of Crocodylus siamensis hemoglobin is antibacterial via a mechanism that likely relies on iron dysregulation and oxidative stress which results in bacterial death. SIGNIFICANCE AND IMPACT OF THE STUDY: We have described for the first time, a novel peptide derived from Crocodylus siamensis hemoglobin hydrolysate, that has the potential to be developed as a novel antimicrobial peptide. This article is protected by copyright. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherWiley Onlineen_US
dc.relation.ispartofseriesJournal of Applied Microbiology;
dc.subjectCrocodylus siamensisen_US
dc.subjecthemoglobin hydrolysateen_US
dc.subjectantibacterial peptideen_US
dc.subjectmembrane permeabilisationen_US
dc.subjectiron dysregulationen_US
dc.subjectoxidative stressen_US
dc.titleA novel antibacterial peptide derived from Crocodylus siamensis hemoglobin hydrolysate induces membrane permeabilisation causing iron dysregulation, oxidative stress and bacterial deathen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1111/jam.13539
dcterms.dateAccepted2017-07-06
rioxxterms.funderCardiff Metropolitan Universityen_US
rioxxterms.identifier.projectCardiff Metropolian (Internal)en_US
rioxxterms.versionAMen_US
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_US
rioxxterms.licenseref.startdate2018-07-16
rioxxterms.freetoread.startdate2018-07-16
rioxxterms.funder.project37baf166-7129-4cd4-b6a1-507454d1372een_US


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