The effect of EPA on inflammatory soluble ICAM- 1 response in a human intestinal cell line
Cardiff Metropolitan University
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Purpose: The aim of this project is to investigate the effects of omega 3 fatty acid EPA 20 μm and 200 μm on human heterogeneous human epithelial colorectal adenocarcinoma cells (CaCo-2 cells) through measuring the amount of soluble ICAM-1 produced by the cells with and without being treated proinflammatory cytokines IL-B, IFNγ. Methods: Cell viability was performed to examine how tolerant CaCo-2 cells are to EPA. CaCo-2 were treated with 200 μm and then doubling dilutions to 6.25 μm of EPA. Then two sandwich ELISA were performed to view one to view the preventative effects of EPA by adding EPA to the samples 1 hour before the proinflammatory cytokines are added and the second sandwich ELISA is to view the therapeutic effects of EPA by adding the proinflammatory cytokines 1 hour before the EPA. 6 different samples will be investigated which include Cells only, Cells + 200 μm EPA and Cells + 20 μm EPA, Cells + Pro + 200 μm EPA, Cells + Pro + 20 μm EPA and Cells + IL-B, IFNγ the therapeutic method also has samples of 1 in 20 dilutions of the samples that contain proinflammatory cytokines. The samples are also left for two different times 6 hours and 24 hours. Results: Cell viability increased with addition of EPA. Results are expressed through tables, bar graphs and are determined statistically significant though two-way and one way ANOVAs. The sandwich ELISA viewing the preventative effects showed no significant difference in samples treated with EPA at concentrations 20 μm or 200 μm compared to the sample without (P>0.05) the same result was viewed in samples with proinflammatory cytokines. There was significant difference between samples of 6 hours and 24 hours in all samples (P<0.05). There was no significant difference in the interaction between the time and sample type (P>0.05). The results with regards to significant difference were the same for difference between sample type time and interaction. One way ANOVAs were performed on therapeutic samples and preventative sample and results showed no Significant difference at 6 hours or 24 hours (P>0.05) Conclusion: Cell viability needs to be repeated. EPA concentrations were not high enough to influence soluble ICAM-1 production therefore no significant difference is observed.
BSc (Hons) Biomedical Science
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