Looking At Effects Of Exosomes on VEGF Secretion by THP Cells For A Proinflammatory Response Possibly Causing A Protumorigenic State
Cardiff Metropolitan University
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Extracellular Vesicles (EVs) are released by many cell types throughout the body. Initially thought to do nothing more than clean up cells, a renewed interest in the field was sparked 30 years due to the discovery of mRNA and proteins within EVs as part of their cargo. This sparked a flurry of research papers, the first extracellular vesicle conference being held and a journal dedicated to EVs being established. A specific area of research within the EV community is the role of exosomes (a subtype of EVs) in the tumour microenvironment and how they are thought to induce a pro-inflammatory response and aid tumour growth. 0.2 AIM The purpose of this study is to look at the effects of exosomes on inflammation and deduce if this is pro or anti-tumourigenic. To this effect, exosomes will be introduced to THP cells to investigate if their addition stimulate the production of VEGF by the THP cells. This will be done in an LPS positive and negative environment. 0.3 METHODS Methods used include the preparation of EVs and THP cells, subsequent protein assays and NanoDrop tests to calculate EV protein concentration and ELISA to measure the levels of VEGF. 0.4 SUMMARY OF RESULTS Conducted over 3 time points up to 48 hours, exosomes did increase the secretion of VEGF by THP cells both in an LPS negative and positive environment, however more so with LPS. 6 0.5 MAIN FINDINGS & CONCLUSIONS Exosomes cause an increase in VEGF production which could account for the high levels of VEGF found in the tumour microenvironment. Research shows VEGF is commandeered by tumours for their own survival as well as VEGF contributing to the inflammation commonly seen with the tumour microenvironment. Therefore, exosomes have a proinflammatory response via their role of intercellular signalling.
BSc (Hons) Biomedical Science
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