Manuka Honey – Investigating its inflammatory effects on adult human dermal fibroblasts (HDFa)
Cardiff Metropolitan University
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Increases in both age expectancy and obesity has led to financial strain and difficulty in treating obesity and age related illness and disease. One such difficulty is in successfully treating prolonged inflammation and chronic wounds. The aim of this research is to identify whether Manuka honey stimulates the Activator Protein 1 (AP-1) transcription factor and proinflammatory cytokine production in adult human dermal fibroblasts (HDFa), by monitoring the levels of soluble intercellular adhesion molecule (sICAM) produced by the HDFa. AP-1 inhibitor concentration needed to be determined via a cell viability assay to ensure HDFa could survive in the presence of the AP-1 inhibitor. The viability assay tested a range of AP-1 inhibitor concentrations up to 10µM, and found that all concentrations tested were viable for use in this research. A series of supernatants were made up for use in a DuoSet ELISA. These included a medium only negative control, ‘HDFa only’ control, HDFa + TNF-α and HDFa + IL-1β positive controls, as well as HDFa + 1% Manuka Honey and HDFa + 1% sugar solution supernatants. Repeats of these supernatants were also made with the inclusion of 1µM and 10µM AP-1 inhibitor. Results of the ELISA found a slight non-significant (P>0.9999) increase with the manuka honey supernatant when compared to the ‘HDFa only’ control, and ICAM production being significantly lower (both P<0.0001 respectively) when compared to TNF-α and IL-1β positive controls. The supernatants containing the AP-1 inhibitor showed a slight non-significant decrease when compared to their non-AP- 1 inhibitor opposites, in all cases except the manuka honey supernatant where the inclusion of the AP-1 inhibitor saw a slight non-significant increase (P<0.9999). While the findings of this research suggest that manuka honey did not stimulate a proinflammatory response within the HDFa, further research using increased concentrations of Manuka honey would be required to ensure this. On top of this, 3 research into the inflammatory effects of manuka honey on other cell types involved in the wound healing process would also need extensive research.
BSc (Hons) Biomedical Science
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