The effect of microvesicles from stored donor blood: an in vitro model to investigate their potential to stimulate production of Interleukin-6 and Interleukin-8 cytokine release from THP-1 cells
Cardiff Metropolitan University
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Studies suggest that aged blood donations cause an adverse effect when transfused to patients. The theory of this derives from evidence of the ‘storage lesion’ where stored erythrocytes are known to release microvesicles, which will accumulate within the donation pack. The study looks at how this accumulation of microvesicles may lead to an adverse effect on a patient due to their potential of being able to exacerbate a pro-inflammatory response. We hypothesised that microvesicles which derived from an aged-blood donation would cause an adverse effect on a patient. The study investigated this hypothesis by measuring the concentration of known pro-inflammatory cytokines IL-6 and IL-8, with the addition of varying volumes of microvesicles, deriving from both fresh and aged-blood, in both the presence and absence of LPS.
BSc (Hons) Biomedical Science
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Melian, Marius (Cardiff Metropolitan University, 2017-06-01)Purpose. The aim of the experiment was to establish whether stored whole blood and erythrocyte blood samples produce a pro or anti inflammatory effect as a consequence of interaction of erythrocyte derived microvesicles ...
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