Hepcidin Gene Expression in THP-1 Monocyte, Macrophage & Dendritic Cells in Response to Synthetic Bacterial & Viral Ligands
Cardiff Metropolitan University
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Hepcidin was isolated at the beginning of the 21st century and is now regarded as the primary peptide responsible for iron homeostasis . Its interaction with FPN leads to its complete internalization and degradation, resulting in decreased iron export from cells  . In addition, hepcidin was also characterized as an AMP by demonstrating weak direct antimicrobial effect against a range of gram negative, gram positive bacteria as well some viral and fungal species  . Its action of iron withholding in cells in considered a source of host defence to limit iron from invading microbes  . Myeloid cells have shown the capacity to induce hepcidin internally without compromising the overall systemic iron levels in a TLR dependent manner  . TLRs are responsible for recognizing pathogen associated molecule that initiate signal transduction pathways leading to an appropriate immune response. A potential immunoregulatory purpose of hepcidin has been suggested in recent years and this investigation will study the ability of THP-1 monocytes, macrophages and dendritic cells to induce hepcidin when challenged by bacterial and viral components, LPS and Poly I:C. In order to warrant further investigation into the effects of hepcidin in this capacity, evidence of hepcidin regulation in response to infection within dendritic cells is required. No conclusive data was gathered from stimulation of monocytes and macrophages with either ligand, which has led to recommended improvements to be made in future studies. Densitometric analysis of dendritic cells indicated that 6h post exposure to LPS and Poly I:C induced a 3.6 and 1.2 fold increase respectively. Conversely, 24h post infection with LPS and Poly I:C, dendritic cells induced a further 1.6 and 5.6 fold increase respectively. The delayed response in hepcidin induction when challenged with bacterial and viral stimulants indicates different pathways directing the impending immune response.
BSc (Hons) Biomedical Science
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Escott, Sophie (Cardiff Metropolitan University, 2010)Hepcidin is an antimicrobial peptide produced in response to inflammation by hepatocytes. It plays a major role in iron homeostasis by decreasing the movement of iron from cells into the plasma. As iron is vital for bacterial ...
Whida, Amal Masre (Cardiff Metropolitan University, 2009)Iron has an important role in the survival of nearly all microorganisms, therefore, one of the strategies used by the immune system in the fight against infection is to reduce iron availability. Thus, iron regulation is ...
Al-Lawati, Hiba Abdul Redha (Cardiff Metropolitan University, 2011)Linking immunity with iron can be well illustrated by the production of hepcidin as it is considered to be the master regulator of iron metabolism in mammals. Iron is an essential element for microbes and higher animals ...