The role of Manuka honey in wound healing and inflammation using IL-6 and human dermal fibroblasts as model systems
Cardiff Metropolitan University
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Introduction In the past, clinical trials have shown that honey helps to accelerate the healing process of chronic wounds due to its antibacterial and anti-inflammatory properties. Over the increasing concern raised by many clinicians because of the burden chronic non-healing wounds have imposed on the healthcare systems, there has been a gap in research of how a nutraceutical, such as Manuka honey, can aid wound healing in a clinical setting. The main aim of this study was to investigate the effects of Manuka honey on the inflammatory process by looking at the production of interleukin-6 (IL-6) cytokine in Human Dermal Fibroblasts (HDFa) cells over a range of Manuka honey concentrations using an Activator Protein-1 (AP-1) inhibitor. Methods Initially a cell viability assay was conducted over a 24-hour and 48-hour period to determine the optimal AP-1 inhibitor concentrations (10uM, 5uM, 2.5uM, 1uM, 0.5uM, 0.25uM, 0.1uM) at which HDFa cells were viable. An (Enzyme-Linked Immunosorbent Assay (ELISA) was performed with samples that were prepared that included HDFa cells, TNF-α, IL-1β, 1.0% Manuka Honey, 1.0% sugar solution and 1uM AP-1 inhibitor. A T-test, ANOVA and Tukey’s multiple comparison statistical analysis was performed on all results that were obtained to understand the significance of the results and form a clear conclusion to the hypothesis. Results For the cell viability assay the results showed that the HDFa cells remained viable over all the AP-1 inhibitor concentrations. The cells favoured higher concentrations (5uM and 10uM) at both incubation times (24-hour and 48-hour). As for the ELISA results, it is clear that IL-1β and TNF-α in combination with the AP-1 inhibitor increased IL-6 production from the HDFa cells. 1.0% Manuka honey produced more IL- 6 than with an AP-1 inhibitor. The 1.0% Manuka honey that was used in this study showed significantly more IL-6 production than from the 1.0% sugar solution (artificial honey). IL-6 reduced dramatically across all samples once the AP-1 inhibitor concentration was increased to 10uM. 5 Conclusion In conclusion, from the data that was obtained and analysed this study has suggested that Manuka honey possess anti-inflammatory properties that can aid wound healing, therefore, potentially could be used to dress wounds to further enhance the healing processes.
BSc (Hons) Biomedical Science
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