To characterise the immunogenicity of stem cell derived neural cells in response to an inflammatory stimulus and flavonoid treatment
Cardiff Metropolitan University
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Neuroinflammation has a severe impact on millions of people around the world via a variety of neurodegenerative diseases and conditions. There are currently very limited treatment options available for these patients, with the primary focus until now being symptom control. Flavonoids however, have been proven to have the ability to regulate the central nervous system, and have been shown to have the potential to slow down both cognitive decline and neurodegeneration in both human and animal models. This research aimed to identify whether treatment with flavonoids could reduce levels of inflammation and cell death in the type of neural cells most commonly affected by neurodegenerative conditions, such as Alzheimer’s, Parkinson’s and Huntington’s disease’. Cell viability assays and a duoset ELISA were carried out, to determine if the flavonoid luteolin had an effect on levels of inflammation in neural cells which were treated with IFN-γ and LPS; if successful, this could then have been applied to the concept of inflammation in patients with neuroinflammatory and degenerative diseases as possible therapeutic treatment. Concentrations of 1ng/ml, 10ng/ml and 100ng/ml of the inflammatory stimuli were used in order to determine the effect this had on cells, so as to compare this to the effect the flavonoid had on cells. Results found that the effect of both inflammatory stimuli and flavonoids had a non-significant effect on either NSCs, astrocytes, or neurons for both the cell viability assays and ELISA procedures, meaning the concentrations were likely insufficient and so did not have a significant effect on the behaviour of the neural cells used. Whilst the findings of this research therefore suggest that the cells were not positively affected by the use of luteolin as a flavonoid, it is possible that with further studies and testing at more concentrations, particularly in cells that had been successfully affected by inflammation, some significant results could be determined; if this was the case, treatment for neuroinflammation could be considered using this flavonoid.
BSc (Hons) Biomedical Science
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