How do Small Colony Variant bacteria of Pseudomonas aeruginosa differentially utilise carbon sources in comparison to the wild type?
Cardiff Metropolitan University
MetadataShow full item record
Introduction Small colony variants (SCV’s) are a genetically and phenotypically unique sub population of bacteria. The most common genetic variations are attributed to mutations in menD, hemB and ctaA1 which collectively leave SCV’s with defective electron transport although other metabolic differences have been documented. Consequently, SCV bacteria are pigment deficient, produce minute colonies, are less susceptible to antibiotic therapy and have distinct biochemical properties. SCV’s are currently believed to be responsible for a number of recurrent infections due to their antibiotic resistant abilities and ability to evade the immune system2 . Methodology A 96 well plate, PM2A by (BioLog, Inc.), pre-coated with a selection of carbon sources was inoculated with Pseudomonas aeruginosa ATCC 9027, to be used as a comparative wild type, and SCV B, SCV G and revertant strains of P. aeruginosa in minimal media, also as provided by Biolog, Inc. specifically for use with PM2A plate. The plates were incubated for 24 hours, with an absorbance reading taken every 15 minutes at 620 nm, at 37 degrees. The revertant used is a reversion of SCV B. Results Heat maps were generated to identify wells in which the carbon sources were differentially utilised. This identified four wells; Acetamide, Glycine, L-leucine and L-Phenylaline that were all found to be utilised differently by SCV’s when compared to growth of the wild type. Following this, comparative growth curves were compiled to analyse growth of all strains in these four wells to better see the extent of differences. Plate pictures are provided to identify SCV’s, in particular their phenotypic features, in comparison to the wild type and revertant. Discussion The four carbon sources mentioned above were utilised differently by SCV’s in comparison to the wild type. The data produced showed that SCV’s were capable of utilising certain carbon sources, such as Glycine, which did not agree with 5 previous findings. Differential utilisation of these carbon sources by SCV’s of P have not been previously described within published literature. Conclusion This study provides preliminary data demonstrating that SCV’s, and their revertant, have unique carbon utilisation profiles. In the future, this could be developed as a means of identifying SCVs in clinical infection and provide more effective treatment plans.
BSc (Hons) Biomedical Science
Showing items related by title, author, subject and abstract.
An Investigation into the Effects of Carbon Black Nanoparticles on Erythrocytes and Leukocytes In Vitro. Smith, Eleanor (University of Wales Institute Cardiff, 2012)The purpose of this laboratory investigation was to discover if the nanoparticle Carbon Black had any significant effect on the membranes of horse erythrocytes and leukocytes, in order to hypothesise the effect of Carbon ...
Swaffield, Luke (Cardiff Metropolitan University, 2013)Carbonated drinks should be seen as a treat to be drunk occasionally, but due to people consuming carbonated drinks as one of their only source of hydration this has raised concerns for obesity problems. The Academy of ...
Holmes, David; Counsell, John; Littlewood, John; Geens, Andrew; Davies, Gareth (2011)This paper introduces a European funded research project to develop design guidance for low carbon and ecological social housing in Wales. From September 2010, all new dwellings in Wales must meet code Level 3, of the Code ...