Modulating the viability and virulence of Staphylococcus psuedintermedius with sub-lethal concentrations of Manuka honey
Cardiff Metropolitan University
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Background: Staphylococcus pseudintermedius is an opportunistic pathogen present in the healthy microbiome of dogs and cats and is a prominent cause of veterinary skin, ear and surgical site infections, displaying zoonotic potential it has also been implicated in human infection. Multidrug resistant strains are commonly isolated during surgery and this study aims to investigate whether medical grade manuka honey has a place as a topical antimicrobial or anti-virulence agent in veterinary infection. Methods: A total of 20 S psuedintermedius strains were used. Antibiotic susceptibility (AST) testing in line with EUCAST disk diffusion method, antibiotics tested: chloramphenicol, penicillin, tetracycline, gentamycin and cefoxatin with and without sub lethal concentrations of manuka honey (5% w/v). Auto agglutination was assessed through suspending a set absorbance (OD6000.09) of cells in PBS with and without 5% (w/v) honey and measuring the % drop in absorbance after incubation at room temperature for 24 hours. The effect of honey on virulence production was determined for both haemolysin and protease using 5% sheep blood agar and 5% skim milk agar with and without 5% honey, a set absorbance (OD6000.09) of cells was suspended in 100ul of soft agar with and without honey and placed into wells bored from the plates. Results: Minimum inhibitory concentrations of manuka honey against all isolates was determined to be < 12% (w/v). An increase in zone size was observed for all isolates during AST testing with some isolates transforming from resistant to susceptible in the presence of honey, auto agglutination was significantly reduced (P<0.05) for all isolates. Haemolysin and protease activity was significantly reduced (P=<0.05) for 12/20 and 10/20 isolates respectively. Conclusion: Manuka honey has antimicrobial activity against S. pseudintermedius isolates. Sub lethal concentrations of manuka honey are effective at down regulating excreted virulence factors from S psuedintermedius isolates and have the potential to improve the efficacy of antibiotics already in use.
Biomedical Science BSc(hons)
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Manuka honey treatment of biofilms of Pseudomonas aeruginosa results in the emergence of isolates with increased honey resistance Camplin, A. L.; Maddocks, Sarah (BioMed Central, 2014)Background Medical grade manuka honeys are well known to be efficacious against Pseudomonas aeruginosa being bactericidal and inhibiting the development of biofilms; moreover manuka honey effectively kills P. aeruginosa ...