Assessment of antimicrobial activity of lemon juice against Acinetobacter baumannii
Cardiff Metropolitan University
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Acinetobacter baumannii is a gram negative pathogen that is a prolific nosocomial infection which causes infections such as cutaneous wound infection, respiratory tract infections, pneumonia, urinary tract infections as well as in rare cases necrotising fasciitis and endocarditis which is a major factor in its recently gained notoriety, and has developed from its recent proliferation around wounded soldiers coming back from the recent desert conflicts of Iraq and Afghanistan; gaining it the nickname ‘iraqibacter’. The aim of this experiment was to prove that Lemon juice has an antimicrobial effect and may have a synergistic effect when used in conjunction with antibiotics to improve their function. Tests were carried out on two bacterial strains of A. baumannii (E75U382259 and NCTC1216) with the former being a resistant strain to antibiotics, and the latter being susceptible to antibiotics. For the investigation of these effects, minimum inhibitory concentration, and minimum bactericidal concentrations were obtained using 96 well microtitre plates. These tests were carried out for gentamicin, lemon juice and a HCl control. Checkerboard assays were also carried out to determine if there was synergy between lemon juice and gentamicin. Results obtained showed that there was a significant difference between the effect of lemon juice and the HCl control with P0.036 &P0.059 respectively. There was a significant difference between the two bacterial strains against gentamicin (P0.00) and no significant difference between the bacterial strain when cultured in lemon juice (P0.721). These results show that lemon juice must act by a different mode of action to gentamicin as there was no significant difference between the two bacterial strains. Therefore, the factors that incur antibiotic resistance in E75U382259 does not provide any tolerance to withstanding lemon juices antimicrobial
Bsc Biomedical Science (Hons)
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