The anti-inflammatory and anti-angiogenic effect of Genistein on MCF-7 breast cancer cell lines
Cardiff Metropolitan University
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The focus of this experiment is to evaluate the anti-inflammatory and anti- angiogenic effects of genistein on MCF-7 breast cancer cell lines. Method: MCF-7 cells, cultured to 80% confluence and separated into 1ml 12 well microtitre plates. TNF-α stimulated the cells into an inflammatory response, to mimic an inflammatory disorder in breast cancer patients. Genistein at high and low concentrations, were added to the cells before and after TNF- α stimulus. This was to compare the effect of genistein as a therapeutic agent and a preventative agent for inflammatory conditions. The cells media was analysed for IL-8 and VEGF, the concentration is compared to a control with no genistein added. Results: Genistein reduced IL-8 and VEGF production by 50% at high concentrations. Particularly under inflammatory stimulus, both as a preventative measure and as a treatment. However at low concentrations, cytokine production upregulated. Conclusion: Genistein can reduce IL-8 and VEGF production at high concentrations in vivo, by downregulating intra cellular pathways and reducing inflammation and angiogenesis. Therefore improving patient prognosis by reducing symptoms of inflammation, metastatic spread and tumour size, via downregulating IL-8 and VEGF expression.
BSc (Hons) Biomedical Science degree
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