The In-vitro effect of Apigenin on Vascular Endothelial Growth Factor and Interleukin-8 production within Caco-2 cells.
Amartey, Jason Otu
Cardiff Metropolitan University
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Background It has been stated that inflammation is the ‘foundation’ of many diseases and disorders. In recent times, there has been a growing interest in the potential of using natural chemicals such as flavonoids as treatment for these sorts of diseases. Apigenin is a flavonoid that is abundant in many fruits and vegetables. Many studies have supported the claim that apigenin has the potential to be used as an anti-inflammatory agent. In this study the flavonoid was studied on its anti-inflammatory and anti-angiogenic effects in caco-2 cells. The aim of this study was to investigate the role of apigenin in reducing the process of inflammation and angiogenesis via the production of VEGF and Interleukin 8. Methodology Apigenin was initially observed on its cell viability effects to help establish a suitable concentration to test throughout the Elisa experiments. 12.5 and 3.125μM of the compound was examined on human epithelial colorectal adenocarcinoma cells (Caco-2) using a Quantikine Duo ELISA to establish the concentration of the inflammatory factors IL-8 and VEGF being secreted by the Caco-2 cells following 6 and 24-hour exposure periods Results Cell viability results revealed that from the concentrations 25μM and above, Apigenin was cytotoxic and had a negative effect on the viability of the cells. There were mixed results with apigenin effect on the production of VEGF and IL-8 in Caco2 cells. In the quantification of VEGF, 12.5μM of Apigenin suppressed the production of VEGF at 4 hours, there was no significant effect of apigenin on VEGF production in Caco2 cells when compared to control cells at the concentration of 3.125 μM In the quantification of IL-8 at 24 hours, there was a calculated significant decrease on the production of IL-8 in the cells (p= 0.0130).at the 3.125μM. 12.5μM of apigenin at 24 hours, increased the production of IL-8 when compared to the control (p= 0.001).
BSc (Hons) Biomedical Science degree
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