To Characterise the Immunogenicity of Stem Cell-derived Neural Cells in Response to an Inflammatory Stimulus and Flavonoid Treatment
Cardiff Metropolitan University
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With increased life expectancies in developed countries neurodegenerative disease (ND) is becoming increasingly widespread and there is an urgent need to derive novel treatment strategies. Human neural stem cells (hNSCs) were differentiated into astrocytes and neurons and analysed using the following protocols: Flow cytometry, DuoSet ELISA and cell viability utilising the flow cytometer. These analyses aimed to elucidate the inflammatory action of lipopolysaccharide (LPS) on neural cells with therapeutic intervention, in the form of the flavonoid chrysin. Flow cytometry did not yield any results, as cells would not conform to single cell suspensions for efficient analysis. ELISA depicted the diminished effect of the inflammatory agent, LPS, to provoke an inflammatory response and induce the cytokine TNF-α, which in turn stunted the potential of chrysin as an anti inflammatory agent. Cell viability shows that each cell type was affected by the initial 1ng dose of LPS, yet failed to elicit any noticeable variation in cell viability when concentrations were increased to 10ng and 100ng. From the analysis undertaken it is inferred that LPS, in this case, elicits the same response independent of the concentrations used. C/EBP-δ is critical for chrysin to produce its beneficial effects as is the TLR4 for the effectiveness of LPS and it is suggested that astrocytes are not a viable target for chrysin due to its supposed inability to modulate the NF-kB pathway. Further analysis of neural cells needs to be conducted by experienced researchers in order to successfully characterise the action and potential of the flavonoid chrysin and the pathways that it inhibits, both in vivo and in vitro in order for it to become a more prominent therapeutic source in the clinic.
(BSc) Biomedical Science
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