Transcriptome Analysis of Megakaryopoiesis Differentiation Process Using Bioinformatics Tools
Alshahrany, Nawal Shaya A
Cardiff Metropolitan University
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Megakaryocytes (MK) are blood cells that give rise to platelets during a process called megakaryopoiesis. Megakaryopoiesis is essential for the proper functioning of haemostasis and alterations in this process can lead to diseases, such as; thrombocytopenia and thrombocytosis. The molecular mechanisms of megakaryopoiesis are poorly understood. Therefore, studies have used immortalised cell lines, such as CHRF-288-11 cells, and microarray analysis to improve the understanding of the molecular mechanisms of megakaryopoiesis. This project is a dry project which involves the use of computer software to manipulate the data generated in a previous research study. This research study (i.e. the wet science) was performed by Fuhrken et al. 1. Using Microsoft Excel, the DAVID bioinformatics tool and String tool, this project will look at what genes are up regulated or down regulated in primary megakaryocytes (PriMK) from day 0 to day 12, it will look to define their functional annotation classification and how these genes interact with each other. The aim of this project is to identify the genes that play an important role in the transcription factor activity and blood coagulation in megakaryopoiesis on day 12. This study identified 11 down-regulated genes and 6 up-regulated genes that play roles in the transcription factor activity on day 12, and 9 up-regulated genes that have roles in the blood coagulation process on day 12.
BSc (Hons) Biomedical Science
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