The effect of Daidzein in modulating an anti-inflammatory immune response in breast cancer.
Cardiff Metropolitan University
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Aim and background: The aim of this study was to determine the role of Daidzein in modulating the immune response in the breast cancer cell line, MDA-MB-231, through assessing its effect on the cytokines IL-6 and IL-8. Studies state that Daidzein has many anti cancer effects, making it ideal for controlling cancer, as has been reported to be a potent ant-inflammatory agent that has the capacity to modulate the crosstalk between the immune cells, as inflammation in a tumor microenvironment results in the secretion of cytokines and growth factors to which the tumor responds positively. Pro-inflammatory cytokines such as the interleukin IL-6 and the chemokine IL-8 are known to regulate the inflammation tumor microenvironment and stimulate cancer cell proliferation and invasion. Hence this study evaluates the effect of Daidzein on the levels of the two pro-inflammatory cytokines, IL-6 and IL-8, in the Breast Cancer Cell line MDA-MB-231, an estrogen negative cell line. Method: MDA-MB-231 cells were firstly used to asses Viability. For viability studies using Daidzein, cells were grown to 80% confluence, detached and seeded at 10000 cells/well into 96well plates. Daidzein at various concentrations and control with media only as negative controls were added. For ELISA, cells were grown to confluence, detached and seeded into 12 well plates at a cell density of 0.5million cells/well before treatment with either TNF alpha or Daidzein at the selected concentrations. 4 Results: Cell viability, aided towards the decision of the different concentration of Daidzein to be used for ELISA, which were 10uM, 6uM and 2uM. ELISA for both IL-8 and IL-6 presented that Daidzein at high concentrations inhibited interleukin expressions. Also additional post treatment with TNF-α expressed better inhibition of interleukins in comparison to pre treatment with TNF-α. Conclusion: Daidzein inhibit IL-6 and IL-8 expression, at high does when post treated with TNF-α for the 24 hour incubation period. However if treated with TNF-α prior to treatment with Daidzein, induces interleukin expression and therefore inflammation.
BSc (Hons) Biomedical Sciences
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