The Inflammatory Effect of Manuka Honey and Methylglyoxal on Primary Human Fibroblast Cells through the Production of Interleukin-6.
Cardiff Metropolitan University
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Introduction: The delayed wound healing process in patients suffering from a diabetic foot ulcer is a serious problem that costs the NHS between £639 million to £662 million a year. The wound healing process is defined by four main phases, which include haemostasis, inflammatory, proliferative and remodelling. The delay in wound healing is caused by an increase of inflammatory cytokines such as IL-6, which leads to chronic inflammation. Many scientists have put forward theories that Manuka honey could be used as treatment as it provides an anti-inflammatory response, however research has shown that infact Manuka honey contains pro-inflammatory properties and causes the stimultation of IL-6. We intend to apply the component MGO, which is an ingredient of MH, to HDFa cells, the amount of IL-6 produced will be measured and will show if MH has a pro-inflammatory effect and whether MGO is responsible for the pro-inflammatory properties of MH. Methods: A twenty-four hour and forty-eight hour cell viability assays were performed on HDFa cells with the addition of MGO. An ELISA was performed to determine the concentration of IL-6 produced in HDFa cells with the addition of various compounds. Statistical analysis of the data was performed by an unpaired two-sample Student’s t-test. P-values <0.05 were considered statistically significant. Results: The ELISA showed that the mean concentration of IL-6 produced by MH 0.5% and MH 1%, 185.60pg/ml and 250.74pg/ml respectively, is significantly higher than the mean concentration of IL-6 produced by the control 18.40pg/ml. However the mean concentration of IL-6 produced by MGO 250μM 10.85pg/ml is significantly lower than the control. Conclusion: In conclusion the results show that MH induces the production of IL-6 in HDFa cells, therefore demonstrating that MH possesses pro-inflammatory properties. However MGO was shown not to be the ingredient of MH that is responsible for inducing the production of IL-6 in HDFa cells. The pro-inflammatory effects could be due to the small concentrations of PPAR ligands present in MH. More research needs to be completed to confirm whether PPAR ligands are responsible of this effect. With the data showing the pro-inflammatory effect MH has, it should be re-evaluated in being used 5 as a treatment for conditions like diabetic foot ulcers as increasing levels of inflammation will lead to increasing the healing time of the diabetic foot ulcer. High levels of IL-6 are also linked to hypertension in patients, which leads to an increased risk of cardiac disease.
BSc Biomedical Science
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