THE CYTOTOXIC EFFECT OF LUTEOLIN, WITH AND WITHOUT KAEMPFEROL, IN SIHA CERVICAL CANCER CELLS
Cardiff Metropolitan University
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The human papillomavirus (HPV) is a highly transmissible virus with over 200 genotypes discovered, categorised based of their risk status. Recurrent infection with the carcinogenic high risk genotypes is responsible for the transformation of cervical epithelium resulting in pre-cancerous lesions and malignant neoplasia. Cervical cancer remains a leading cause of death among women worldwide despite implemented screening programmes, approved vaccines and established treatment in developed countries. The lack of clinical expertise and health care funding in developing regions is a major factor accounting for the mortality of cervical cancer. Consequently, inexpensive and readily accessible alternatives to cervical cancer prevention and treatment are undergoing investigation. Luteolin and kaempferol are botanical agents which are common constituents of fruit and vegetable products and contribute to the human diet. Both agents have been extensively investigated for their beneficial biological functions, predominantly their ability to exert cancer-specific cytotoxicity. In the present study, CellTiter- Blue viability assays and intracellular flow cytometry were used to determine the potential ability of luteolin, with and without kaempferol, in exerting a dose and time dependent cytotoxicity in the cervical cancer cell line, SiHa, through the upregulation of p53. Findings of this study revealed that luteolin induced cytotoxicity within SiHa cells in a dose and time dependent manner (p<0.05) whereas kaempferol exerted a dose-dependent cytotoxic response (p<0.05). For both botanical agents, there was a confirmed interaction between agent dose and exposure time (p<0.05). Furthermore, it was revealed that 75μM of luteolin induced a significant increase in intracellular p53 expression (p<0.05) however 50μM of luteolin generated a significant down-regulation of p53 (p<0.05). Reductions in SiHa cell viability at this concentration are possibly attributed to an apoptotic mechanism independent of p53. 50μM of kaempferol was successful in potentiating the effect of luteolin at 50μM generating a significant up-regulation of p53 (p<0.05) in comparison to the p53 control and luteolin administration alone (p>0.05).
BSc Biomedical Science
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