Manuka Honey: It’s Role in Wound Healing and Inflammation
Cardiff Metropolitan University
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Since time immemorial the health value of manuka honey has been universally acknowledged. Manuka honey exhibits an array of biological properties ranging from anti-oxidant, anti-inflammatory, anti-biotic and wound healing to immune-stimulatory responses. Approximately 5-8% of diabetic patients suffer from chronic foot ulcers as a result of impaired wound healing. Chronic diabetic ulcer sufferers exhibit increased levels of apoptosis within their infected tissues which is a leading cause of inefficient wound healing (Edmands and Foster, 2006). The purpose of this research study was to determine the in-vitro response of human dermal fibroblasts (HDFa) critical to the wound healing process when supplemented with Manuka Honey and Methylglyoxal, a bioactive compound responsible for exertingManuka Honey’s antimicrobial properties. HDFa cells were incubated in Dulbecco’s Modified Eagle Medium (DMEM) in the presence and absence of two organically extracted Manuka Honey concentrations, two known concentrations of Sugar solutions, and two know concentrations of Methylglyoxal, as well as TNFα and IL-IB for 24 hours. The supernatants were poised and underwent analysis for expression Interleukin-8 (IL-8) through the process of a standard ELISA. Resultsmeasured the amount of cytokine IL-8 released when cells were supplemented with the previously mentioned concentrations and moreover were analysed relative to cells only. The data gathered was normalised to cells only (100%). The biological activity of the solutions combined with HDFa cells demonstrated significant variation of pro-inflammatory and anti-inflammatory activity. The responses established activations of multiple molecular pathways, dose response relationships and mechanisms of action.
BSc Biomedical Science
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