Investigating the cytokine induced inflammatory effects of carbon black particulate matter in cultured human monocyte cell models.
Cardiff Metropolitan University
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Introduction: A strong positive correlation between particulate matter exposure and cardiovascular mortality and morbidity has been previously observed. Epidemiological and toxicological studies have observed a PM-induced alteration in human monocytes post long- and short-term exposure, but there has currently been little explanation of the putative mechanisms involved Methodology: THP-1 human monocyte cell lines were incubated with different concentrations of SRM 1648a Carbon Black pollution particles for 24-hours to measure the dose-dependent effect. ELISA analysis of the sample supernatant indicated the concentration of TNF-α in solution. Results: TNF-α secretion from THP-1 cells was induced in a dose-dependent matter post 24-hour incubation. Data was statistically significant (<0.001) for both, the standard and unknown samples. Microscopic cell imaging presented more morphological abnormalities as PM-exposure increased. Discussion: Inhalation of PM can alter monocytes, both genotypically and phenotypically causing them to function abnormally. Through a variety of putative mechanisms, the rate of atherosclerotic plaque can be accelerated which can increase the chance of a CVD event. Conclusion: Inhalation of particulate matter aggravates human monocytes which can then detrimentally influence the risk of CVD.
BSc (Hons) Biomedical Sciences (Health, Excercise & Nutrition)
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