The Effect of an Organic Extract of Manuka Honey on Angiogenesis via the Akt Signalling Pathway
Cardiff Metropolitan University
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Angiogenesis and inflammation are two key regulatory processes involved in chronic disease including cancer and cardiovascular disease (CVD) (Kim et al., 2013). Protein kinase B (Akt) is a serine/threonine protein kinase involved in many cellular processes including angiogenesis. The regulation of Akt is modulated by the phosphorylation of a number of different protein kinases including phosphoinositide 3-kinase (P13K), phosphoinositide-dependant kinase-1 (PDK-1) and the mechanistic target of rapamycin (mTOR), leading to a signalling cascade. Dysregulation of the Akt signalling pathway is a common pathological process involved in cancer and other chronic disease such as CVD, diabetes and neurological diseases (Hers et al., 2011). Diet based therapies such as the use of flavonoids have been seen to target such diseases as they express anti-inflammatory and anti-angiogenic properties (Goya et al., 2016; Tian et al., 2014). Manuka honey has been widely reported as being beneficial to health promotion, in which flavonoid components identified within Manuka honey have been reported to have therapeutic effects (Moniruzzaman et al., 2013). This study aimed to investigate the effect of an organic extract of Manuka honey (ME) on angiogenesis via the Akt signalling pathway in cultured human umbilical vein endothelial cells (HUVEC) cells. The findings of this study found ME upregulated target-specific antibodies related to the Akt signalling pathway including proline-rich Akt substrate 40kDa (PRAS40) and 5’ AMP-activated protein kinase alpha (AMPK-), however down regulated phosphatase and tensin homolog (PTEN) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) compared to untreated HUVEC cells (control). Conclusively the methods and findings indicate that this paper was a pilot study, thus further research needs to be carried out taken from the these findings in order to clarify the action of ME in Akt signalling and angiogenesis.
BSc (Hons) Biomedical Sciences (Health, Excercise and Nutrition)
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