Interaction between Staphylococcus aureus and Pseudomonas aeruginosa is beneficial for colonisation and pathogenicity in a mixed-biofilm

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Author
Alves, Patricia M.
Al-Badi, Eide
Withycombe, Cathryn
Jones, Paul M.
Purdy, Kevin J.
Maddocks, Sarah
Date
2018-01-12Acceptance date
2018-01-09
Date Deposited
2018-01-23
Type
Article
acceptedVersion
Publisher
Oxford University Press
ISSN
2049-632X
Metadata
Show full item recordAbstract
Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early (<24h) biofilm formation, and interaction with human keratinocytes. We observed that S. aureus predominated in biofilm and non-attached bacterial aggregates, acting as a pioneer for the attachment of P. aeruginosa. We report for the first time that S. aureus mediates a significant (P<0.05) increase in the attachment of P. aeruginosa to human keratinocytes, and that P. aeruginosa promotes an invasive phenotype in S. aureus. We show that co-infected keratinocytes exhibit an intermediate inflammatory response concurrent with impaired wound closure that is in keeping with a sustained pro-inflammatory response which allows for persistent microbial colonisation. These studies demonstrate that, contrary to the dominant hypothesis, interactions between S. aureus and P. aeruginosa may be an important factor for both colonisation and pathogenicity in the chronic infected wound.
Journal/conference proceeding
Pathogens and Disease;
Citation
Alves, P.M., Al-Badi, E., Withycombe, C., Jones, P.M., Purdy, K.J. and Maddocks, S.E. (2018) 'Interaction between Staphylococcus aureus and Pseudomonas aeruginosa is beneficial for colonisation and pathogenicity in a mixed-biofilm', Pathogens and Disease. https://doi.org/10.1093/femspd/fty003
Description
This article was published in Pathogens and Disease on 12 January 2018 (online), available at https://doi.org/10.1093/femspd/fty003
Sponsorship
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
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