Surface Finish has a Critical Influence on Biofilm Formation and Mammalian Cell Attachment to Additively Manufactured Prosthetics
MetadataShow full item record
Additive manufacturing (AM) technologies enable greater geometrical design freedom compared with subtractive processes. This flexibility has been used to manufacture patient-matched implants. Although the advantages of AM are clear, the optimization at each process stage is often understated. Here we demonstrate that surface finishing of selective laser melted (SLM) implants significantly alters topography, which has implications for cellular and biofilm adhesion. Hot isostatic pressing of as-fabricated Ti-6Al-4V implants was shown to reduce porosity (1.04 to 0.02%) and surface roughness (34 ± 8 to 22 ± 3 μm). Despite these surface changes, preosteoblasts exhibited a similar viability and proliferation after 7 days of culture. Contrastingly, sandblasting and polishing significantly reduced cellular activity and increased cytotoxicity. Bacterial specimens (Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa) adhered more homogeneously to sandblasted implants compared with other treatments. This suggests that sandblasting may place the implant at risk of infection and reduce the strength of interaction with the surrounding soft tissues. The ability to tune the adhesion of cells to additively manufactured Ti-6Al-4V implants using postprocessing methods was demonstrated. Because the degree of tissue integration required of implants is application specific, these methods may be useful to tailor osseointegration. However, surface competition between mammalian and bacterial cells remains a challenge
ACS Biomaterials Science and Engineering;
Cox, S.C., Jamshidi, P., Eisenstein, N.M., Webber, M.A., Burton, H., Moakes, R.J., Addison, O., Attallah, M., Shepherd, D.E. and Grover, L.M. (2017) 'Surface Finish has a Critical Influence on Biofilm Formation and Mammalian Cell Attachment to Additively Manufactured Prosthetics', ACS Biomaterials Science & Engineering, 3(8), pp.1616-1626.
This article was published in ACS Biomaterials Science & Engineering on 12 June 2017 (online) available at http://dx.doi.org/10.1021/acsbiomaterials.7b00336
Showing items related by title, author, subject and abstract.
Hyperspectral chemical imaging reveals spatially varied degradation of polycarbonate urethane (PCU) biomaterials Dorrepaal, R.M.; Lawless, B.M.; Burton, Hanna; Espino, D. M.; Shepherd, D.E.T.; Gowen, A.A. (Elsevier, 2018-04-05)Hyperspectral chemical imaging (HCI) is an emerging technique which combines spectroscopy with imaging. Unlike traditional point spectroscopy, which is used in the majority of polymer biomaterial degradation studies, HCI ...
Eggbeer, Dominic; Bibb, Richard; Evans, P. (Matrix Marketing, 2006-12-01)Previous research into the application of digital technologies in maxillofacial prosthetics has focussed on the creation of overall shape, but very little research had explored the incorporation of implant retention ...
Peel, Sean; Eggbeer, Dominic (2016)Purpose: The technical efficacy of, and clinical benefits from using Computer Aided Design (CAD) and Additive Manufacturing (AM) in the production of patient-specific devices (implants and guides) has been established. ...