• English
    • Welsh
  • English 
    • English
    • Welsh
  • Login
Search DSpace:
  • Home
  • Research at Cardiff Met
  • Library Services
  • Contact Us
View item 
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Health and Risk Management
  • View item
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Health and Risk Management
  • View item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Exposure of Escherichia coli to human hepcidin results in differential expression of genes associated with iron homeostasis and oxidative stress

Thumbnail
View/open
Author's post-print (640.4Kb)
Author
Pascoe, Michael J.
Lueangsakultha, Jiraporn
Ripley, Delia
Morris, Keith
Maddocks, Sarah
Date
2018-04-06
Acceptance date
2018-04-05
Type
Article
Publisher
Oxford University Press
ISSN
0378-1097
1574-6968 (online)
Embargoed until
2019-04-06
Metadata
Show full item record
Abstract
Hepcidin belongs to the antimicrobial peptide family but has weak activity with regards to bacterial killing. The regulatory function of hepcidin in humans serves to maintain an iron-restricted environment that limits the growth of pathogens; this study explored whether hepcidin affected bacterial iron homeostasis and oxidative stress using the model organism Escherichia coli. Using the Miller assay it was determined that under low iron availability exposure to sub-inhibitory doses of hepcidin (4 -12μM) led to 2-fold and 4-fold increases in the expression of ftnA and bfd respectively (p<0.05), in both a wild type (WT) and ∆fur (ferric uptake regulator) background. Quantitative real-time PCR analysis of oxyR and sodA, treated with 4 or 8µM of hepcidin showed that expression of these genes was significantly (p<0.05) increased, whereas expression of lexA was unchanged, indicating that hepcidin likely mediated oxidative stress but did not induce DNA damage.
Journal/conference proceeding
FEMS Microbiology Letters;
Citation
Pascoe, M.J., Lueangsakulthai, J., Ripley, D., Morris, R.H. and Maddocks, S.E. (2018) 'Exposure of Escherichia coli to human hepcidin results in differential expression of genes associated with iron homeostasis and oxidative stress', FEMS microbiology letters, 365 (10). https://doi.org/10.1093/femsle/fny089
URI
http://hdl.handle.net/10369/9579
DOI
https://doi.org/10.1093/femsle/fny089
Description
This article was published in FEMS microbiology letters on 06 April 2018 (online), available at https://doi.org/10.1093/femsle/fny089
Sponsorship
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
Collections
  • Health and Risk Management [200]

Related items

Showing items related by title, author, subject and abstract.

  • Thumbnail

    TLR- and NOD - dependent hepcidin expression by HaCaT keratinocytes 

    Al-Bahlouli, Hamed Mohammed (Cardiff Metropolitan University, 2011)
  • Thumbnail

    TLR-dependent hepcidin expression by THP-1 cells in response to TLR4 and TLR9 (LPS and CpG) 

    Al-Lawati, Hiba Abdul Redha (Cardiff Metropolitan University, 2011)
    Linking immunity with iron can be well illustrated by the production of hepcidin as it is considered to be the master regulator of iron metabolism in mammals. Iron is an essential element for microbes and higher animals ...
  • Thumbnail

    TLR-dependent hepcidin expression by THP-1 cells in response to LPS and poly I:C 

    Al Shuraiqi, Rajaa (Cardiff Metropolitan University, 2011)
    Hepcidin a hepatic peptide hormone mainly produces by the liver tissue, it is also produce in a small amount by other tissue including lung, heart and macrophages. Hepcidin plays a critical role in iron regulation and ...

Browse

DSpace at Cardiff MetCommunities & CollectionsBy issue dateAuthorsTitlesSubjectsThis collectionBy issue dateAuthorsTitlesSubjects

My Account

Login

Statistics

Most Popular ItemsStatistics by CountryMost Popular Authors

DSpace software copyright © 2002-2015  DuraSpace
Contact us | Send feedback | Administrator