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dc.contributor.authorHassan, Nurudeen
dc.contributor.authorAli, Ahmed
dc.contributor.authorWithycombe, Cathryn
dc.contributor.authorAhluwalia, Maninder
dc.contributor.authorAl-Nasseri, Raya Hamdan
dc.contributor.authorTonks, Alex
dc.contributor.authorMorris, Keith
dc.date.accessioned2018-05-08T13:56:23Z
dc.date.available2018-05-08T13:56:23Z
dc.date.issued2018-03-19
dc.identifier.citationHassan, N., Ali, A., Withycombe, C., Ahluwalia, M., Al-Nasseri, R.H., Tonks, A. and Morris, K. (2018) 'TET-2 up-regulation is associated with the anti-inflammatory action of Vicenin-2', Cytokine, 108, pp.37-42.en_US
dc.identifier.issn1043-4666
dc.identifier.issn1096-0023
dc.identifier.urihttp://hdl.handle.net/10369/9608
dc.descriptionThis article was published in Cytokine on 19 March 2018 (online), available at https://doi.org/10.1016/j.cyto.2018.03.016en_US
dc.description.abstractVicenin-2, a C-glycoside flavone that is present in many plant sources, exerts potent anti-inflammatory effects in a number of cell and animal models of inflammation. Ten-eleven translocation (TET)-2 has recently gained considerable attention due to the role it plays in regulating the inflammasome. We studied the ability of Vicenin-2 (V-2) to regulate a range of lipopolysaccharide (LPS) stimulated inflammatory activities in PMA-differentiated THP-1 cells and human primary mononuclear cells. We also investigated the action of V-2 on the secretion of NLRP3 inflammasome regulated cytokines (IL-1β and IL-18) by ELISA, and determined if V-2 can regulate the expression of NLRP3, IL-10, IL-1Ra and TET-2. The effect of V-2 on NF-κB signalling was investigated by fluorescence microscopy and gene reporter assay. Additionally, the effect of V-2 on LPS-induced phosphorylation of IKB-α was also investigated by Western blot analysis. V-2 down-regulated LPS-induced secretion of proinflammatory cytokines (TNF-α and IL-1β), in both THP-1 and primary mononuclear cells. V-2 also decreased the LPS-stimulated secretion of IL-18 in THP-1 cells. V-2 significantly down-regulated TNF-α induced NF-κB reporter activity in HEK293T transfected cells and attenuated IKB-α phosphorylation in THP-1 cells. V-2 treatment also induced enhanced nuclear staining of the p50 subunit and reduced p65 subunit of NF-κB. V-2 treatment alone increased the expression of anti-inflammatory cytokine, IL-10, and the regulator of the inflammasome; IL-1Ra, in the presence of LPS. V-2 also significantly decreased LPS-induced NLRP3 expression while concomitantly increasing TET-2 expression. This study demonstrates that the anti-inflammatory actions of V-2 are associated not only with increased IL-10 and IL-1Ra expression, but also with TET-2 up-regulation. Further work is required to establish if the effects of V-2 can be definitively linked to TET-2 activity and that these actions are mirrored in a range of relevant cell types.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesCytokine;
dc.subjectTET-2en_US
dc.subjectmacrophagesen_US
dc.subjectinflammasomeen_US
dc.subjectNF-κBen_US
dc.subjectVicenin-2en_US
dc.titleTET-2 up-regulation is associated with the anti-inflammatory action of Vicenin-2en_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1016/j.cyto.2018.03.016
dcterms.dateAccepted2018-03-14
rioxxterms.funderCardiff Metropolitan Universityen_US
rioxxterms.identifier.projectCardiff Metropolian (Internal)en_US
rioxxterms.versionAMen_US
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_US
rioxxterms.licenseref.startdate2019-03-19
dc.refexceptionOA compliant
rioxxterms.freetoread.startdate2019-03-19
rioxxterms.funder.project37baf166-7129-4cd4-b6a1-507454d1372een_US


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