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dc.contributor.authorDuckworth, Peter F.
dc.contributor.authorRowlands, Richard S.
dc.contributor.authorBarbour, Michele E.
dc.contributor.authorMaddocks, Sarah
dc.date.accessioned2018-07-24T09:12:16Z
dc.date.available2018-07-24T09:12:16Z
dc.date.issued2018-07-19
dc.identifier.citationDuckworth, P.F., Rowlands, R.R., Barbour, M.E. and Maddocks, S.E. (2018) 'A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings', 215(Oct), 141-147en_US
dc.identifier.urihttp://hdl.handle.net/10369/9694
dc.descriptionThis article was published in Microbiological Research available at https://doi.org/10.1016/j.micres.2018.07.009en_US
dc.description.abstractSeveral models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments. Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface. In this manuscript, a new biofilm flow device for study of wound biofilms is reported. The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings. Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8–10 colony forming units (CFU). There was a 3–4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesMicrobiological Research;
dc.subjectbiofilmsen_US
dc.subjectwound infectionen_US
dc.subjectefficacyen_US
dc.subjectwound dressingsen_US
dc.titleA novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressingsen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1016/j.micres.2018.07.009
dcterms.dateAccepted2018-07-18
rioxxterms.versionAMen_US
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden_US
rioxxterms.licenseref.startdate2018-07-24
rioxxterms.freetoread.startdate2019-07-19


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