Effect of glutaraldehyde based cross-linking on the viscoelasticity of mitral valve basal chordae tendineae

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Author
Constable, M.
Burton, Hanna
Lawless, B.M.
Gramigna, V.
Buchan, K.G.
Espino, D.M.
Date
2018-07-13Acceptance date
2018-07-05
Type
Article
Publisher
Springer Nature
ISSN
1475-925X
Metadata
Show full item recordAbstract
Background
Mitral valve failure can require repair or replacement. Replacement bioprosthetic valves are treated with glutaraldehyde prior to implantation. The aim of this study was to determine the changes in mechanical properties following glutaraldehyde fixation of mitral valve chordae.
Methods
To investigate the impact of glutaraldehyde on mitral valve chordae, 24 basal chordae were dissected from four porcine hearts. Anterior and posterior basal (including strut) chordae were used. All 24 chordae were subjected to a sinusoidally varying load (mean level 2N, dynamic amplitude 2N) over a frequency range of 0.5–10 Hz before and after glutaraldehyde treatment.
Results
The storage and loss modulus of all chordal types decreased following glutaraldehyde fixation. The storage modulus ranged from: 108 to 119 MPa before fixation and 67.3–87.4 MPa following fixation for basal chordae; 52.3–58.4 MPa before fixation and 47.9–53.5 MPa following fixation for strut chordae. Similarly, the loss modulus ranged from: 5.47 to 6.25 MPa before fixation and 3.63–4.94 MPa following fixation for basal chordae; 2.60–2.97 MPa before fixation and 2.31–2.93 MPa following fixation for strut chordae.
Conclusion
The viscoelastic properties of mitral valve chordae are affected by glutaraldehyde fixation; in particular, the reduction in storage moduli decreased with an increase in chordal diameter.
Journal/conference proceeding
BioMedical Engineering OnLine;
Citation
Constable, M., Burton, H.E., Lawless, B.M., Gramigna, V., Buchan, K.G. and Espino, D.M. (2018) 'Effect of glutaraldehyde based cross-linking on the viscoelasticity of mitral valve basal chordae tendineae', Biomedical Engineering Online, 17(1), p.93.
Description
Article published open access in Biomedical Engineering Online available at https://doi.org/10.1186/s12938-018-0524-2
Sponsorship
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
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